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MetabolomicsThe in-gel proteomic characterization of exercise-induced sweat - (part III)
Not available
ORGANISM(S): Homo sapiens 
ST001072 | MetabolomicsWorkbench
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsUpdated in-gel digestion for protein analysis by mass spectrometry
The in-gel digestion of proteins for analysis by liquid chromatograph mass spectrometry has been used since the early 1990s. Although several improvements have contributed to increasing the quality of the data obtained, many recent publications still use sub-optimal approaches. We present an updated...
ORGANISM(S): Homo sapiens (Human) 
2018-10-02 | PXD009600 | Pride
Proteomics Mass spectrometry In-gel digestion Trypsin 
TranscriptomicsSystems Biology Analysis of Tenofovir 1% Gel in a Phase I Rectal Microbicide Trial
In MTN-007, a phase 1, randomized, double-blinded rectal microbicide trial, we used systems genomics/proteomics to determine the effect of tenofovir 1% gel, nonoxynol-9 2% gel, placebo gel or no treatment on rectal biopsies taken at baseline, after one application or after seven daily applications (...
ORGANISM(S): Homo sapiens 
2015-02-23 | E-GEOD-57025 | biostudies-arrayexpress
ProteomicsComparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer
Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD009860 | Pride
Mass spectrometry Breast cancer Breast milk 2d-page Proteomics Biomarkers 
GenomicsCompound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.
Compound treatment in the alveolar epithelial spheroids derived from the dual reporter iPSC in "On-Gel" culture.
PRJNA1007705 | ENA
ProteomicsDirect in-gel profiling of protease specificity (DIPPS)
Determination of protease specificity is of crucial importance for understanding protease function. We have developed the first gel-based label-free proteomic approach (DIPPS) that enables quick and reliable determination of protease cleavage specificities under large variety of experimental conditi...
ORGANISM(S): Aeropyrum pernix Homo sapiens (Human) 
2017-07-06 | PXD004218 | Pride
Specificity profiling Protease Legumain Cathepsin Degradomics 
GenomicsIdentification of differentially expressed genes in actinic keratosis samples treated with ingenol mebutate gel
Identification of differentially expressed genes in actinic keratosis samples treated with ingenol mebutate gel
PRJNA595920 | ENA
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