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ProteomicsConformational rearrangements upon start codon recognition in human 48S translation initiation complex
Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD031985 | Pride
Ribosome 48s initiation comples Eif1 
TranscriptomicsTranscription Initiation Platforms and GTF recruitment at tissue-specific enhancers and promoters
Recent works have shown that RNA Polymerase (Pol) II can be recruited to, and transcribe, distal regulatory regions. Here, we analyzed transcription initiation and elongation through genome-wide localization of Pol II, General Transcription Factors (GTFs) and active chromatin in developing T-cells. ...
ORGANISM(S): Mus musculus 
2011-05-20 | E-GEOD-29362 | biostudies-arrayexpress
TranscriptomicsStably-paused genes revealed through inhibition of transcription initiation by the TFIIH inhibitor Triptolide
Transcription by RNA Polymerase II (Pol II) in metazoan is regulated in several steps, including preinitiation complex (PIC) formation, initiation, Pol II escape, productive elongation, cotranscriptional RNA-processing and termination. Genome-wide studies have demonstrated that the phenomenon of ...
ORGANISM(S): Homo sapiens 
2015-01-05 | E-GEOD-63314 | biostudies-arrayexpress
GenomicsOrchestration of Floral Initiation by APETALA1
The MADS-domain transcription factor APETALA1 (AP1) is a key regulator of Arabidopsis flower development. To understand the molecular mechanisms underlying AP1 function, we identified its target genes during floral initiation using a combination of gene expression profiling and genome-wide binding s...
ORGANISM(S): Arabidopsis thaliana 
2010-04-02 | E-GEOD-20176 | biostudies-arrayexpress
GenomicsIdentification of the primary target of the chemotherapeutic agent CX-5461 as a late step in transcription initiation by RNA Polymerase I
In recent years, several small molecule cytotoxic drugs have been identified as potential inhibitors of ribosome biogenesis (Drygin et al., 2011; Peltonen et al., 2014a; Peltonen et al., 2014b). CX-5461 is one such drug that has also demonstrated anticancer potential for a wide range of malignancies...
ORGANISM(S): Mus musculus 
2021-04-07 | E-MTAB-9242 | biostudies-arrayexpress
TranscriptomicsEffect of low-Tw treatment before panicle initiation on the chilling response of spikelets at the booting stage in rice.
In rice (Oryza sativa L.), chilling-induced male sterility increased when plants experienced low water temperature (Tw, 18 M-BM-0C for 14 days) before panicle initiation. The number of mature pollen grains after chilling at the booting stage (12 M-BM-0C for 5 days) was only approximately 45% of tota...
ORGANISM(S): Oryza sativa 
2014-12-22 | E-GEOD-59221 | biostudies-arrayexpress
TranscriptomicsGlucose depletion inhibits translation initiation in yeast - polysome and monosome profiling
This is a polysome/monosome profiling study on yeast undergoing glucose depletion. Polysomes and monosomes are extracted and the transcripts bound to each fraction are measured by microarray. The study reveals that glucose depletion inhibits translation initiation in a mechanism involving eIF4A loss...
ORGANISM(S): Saccharomyces cerevisiae 
2011-11-11 | E-MEXP-3038 | biostudies-arrayexpress
TranscriptomicsRNA-seq GLV6 overexpression during lateral root initiation in Arabidopsis thaliana
The goal of this experiment was to identify the downstream targets of the GOLVEN6 peptide signaling pathway in Arabidopsis thaliana, specifically during lateral root initiation. Using an estradiol inducible GLV6 overexpression construct in wildtype and rgi1rgi5 double mutant (mutant in receptors for...
ORGANISM(S): Arabidopsis thaliana 
2022-02-13 | E-MTAB-10891 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis polysomal and non-polysomal RNA samples isolated from 10-day-old wild-type and eif3h mutant plants
Microarray comparisons of polysome loading in wild-type Arabidopsis and eif3h mutant; Goal: ; To find the target mRNAs that are translationally regulated by eIF3h. BACKGROUND: The eukaryotic translation initiation factor eIF3 has multiple roles during the initiation of translation of cytoplasmic mRN...
ORGANISM(S): Arabidopsis thaliana 
2008-06-14 | E-GEOD-6024 | biostudies-arrayexpress
ProteomicsDAP5 directs a widespread alternate form of cap-dependent mRNA translation initiation using eIF3d for specialized cell functions
Affinity purification of eIF4GII, eIF4GI and DAP5 using a HA tag on the N-terminus of each protein.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-05-25 | MSV000082407 | MassIVE
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