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ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsAn automated intact glycopeptide enrichment method for serum site-specific N-glycoproteomic analysis
We developed an automated glycopeptide enrichment method for the analysis of serum site-specific N-glycoproteome. This automated method allowed for processing one sample within 20 min. It showed higher enrichment specificity, more intact glycopeptide identifications, and better quantitative reproduc...
ORGANISM(S): Homo Sapiens (human) 
2021-04-06 | PXD025151 | JPOST Repository
ProteomicsEffective Enrichment Strategy Using Boronic Acid Functionalized Mesoporous Graphene Silica Composites for Intact Glycopeptide Analysis in Human Serum
The heterogeneity and low abundance of protein glycosylation present challenging barriers to the analysis of intact glycopeptides, which is key to comprehensively understanding the role of glycosylation in an organism. Efficient and specific enrichment of intact glycopeptides could help greatly with...
ORGANISM(S): Human 
2021-04-28 | MSV000087324 | MassIVE
ProteomicsMALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening
To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze / thaw cycles and to reduce high costs for shipping and storage, we sought to dev...
ORGANISM(S): Homo sapiens (Human) 
2021-05-20 | PXD024669 | Pride
Intact serum protein analysis Maldi-ms profiling Fast frozen serum Serum proteolysis analysis Dried serum spots 
ProteomicsGlycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis
Glycosylation is an important post-translational modification (PTM) mechanism of proteins, that modulates protein function. Measurement of clinically adopted cancer protein biomarkers is commonly performed using affinity-based techniques, which often suffer from poor specificity and inability to dis...
ORGANISM(S): Homo sapiens (Human) 
2023-01-11 | PXD038602 | Pride
Intact proteins Hepatocellular carcinoma 
ProteomicsOrbitrap mass spectrometry and high-field asymmetric waveform ion mobility spectrometry (FAIMS) enable the indepth analysis of human serum proteoforms
In this study, we describe a straightforward protocol for intact proteoform sample preparation based on depletion of albumin and immunoglobulins followed by simplified fractionation of remaining serum proteins via polyacrylamide gel electrophoresis. After molecular weight-based fractionation, we sup...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-02 | MSV000092574 | MassIVE
MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
ProteomicsGlycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis
Alpha fetoprotein (AFP) is a circulating cancer biomarker implicated in multiple neoplastic malignancies, including hepatocellular carcinoma (HCC). AFP glycoforms with core fucosylation structure (AFP-L3) are used clinically as a biomarker, to predict risk of developing HCC and to monitor its recur...
ORGANISM(S): Homo sapiens (Human) 
2023-01-11 | PXD038606 | Pride
Afp-l3 proteforms Core fucose Fucosylation High-resolution mass spectrometry Intact protein analysis 
ProteomicsIntact N-glycopeptides in Serum Haptoglobin between HCC and Cirrhosis
A workflow for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis has been developed.
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD011239 | Pride
Human Serum Haptoglobin Lc-ethcd-ms/ms Liver 
ProteomicsLarge scale intact glycopeptide identification by Mascot database search
We present the adaptability of Mascot search engine for automated identification of intact glycopeptide mass spectra. The steps involved in adopting Mascot for intact glycopeptide analysis include: i) assigning unique one letter codes for monosaccharides, ii) linearizing glycan sequences and iii) pr...
ORGANISM(S): Homo sapiens (Human) 
2018-02-08 | PXD005931 | Pride
Mascot Glycoproteomics Higher-energy collisional induced dissociation (hcd) Prostate cancer Serum 
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