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We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nano-scale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were firstly enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed wit...
ORGANISM(S): Homo Sapiens (human) 
Quantitative metaproteomics is a relatively new research field by applying proteomics technique to study microbial proteins of microbiome, and holds the great potential to truly quantify the functional proteins actually expressed by microbes in the biological environment such as gastrointestinal tra...
ORGANISM(S): Mus musculus (Mouse) 
2018-12-17 | PXD011735 | Pride
Despite recent advances in MS-based proteomics, comprehensive phosphoproteomics analysis of small populations of cells remains a daunting task, due primarily to the general lack of sufficient MS signals from such limited amounts of samples. In light of the unique multiplexing feature of isobaric lab...
ORGANISM(S): Homo Sapiens (human) 
Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-08-23 | MSV000084230 | MassIVE
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
Recent developments in mass spectrometry-based single-cell proteomics (SCP) have resulted in dramatically improved sensitivity, yet the relatively low measurement throughput remains a limitation. Isobaric and isotopic labeling methods have been separately applied to SCP to increase throughput throug...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD040455 | Pride
Top-down mass spectrometry provides a powerful approach for analyzing and quantifying intact proteoforms, i.e., the distinct molecular forms of proteins. Isobaric labeling-based quantification strategies offer the advantages of multiplexing and increased analytical depth. However, a major challenge ...
ORGANISM(S): Bos taurus (Bovine) Gallus gallus (Chicken) 
2026-06-08 | PXD074730 | Pride
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