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ProteomicsAnalysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT)
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614) 
2016-03-17 | MSV000079594 | MassIVE
UnknownPeptide labeling with isobaric tags yields higher identification rates using iTRAQ 4-plex compared to TMT 6-plex and iTRAQ 8-plex on LTQ Orbitrap.
Not available
S-EPMC3093924 | biostudies-literature
ProteomicsRelative quantitative analysis of proteome based on iTRAQ/TMT
Relative quantitative analysis of proteome based on iTRAQ/TMT.
ORGANISM(S): Homo sapiens (Human) 
2026-08-18 | PXD054529 | Pride
Itraq/tmt Quantitative analysis 
UnknownIsoProt: A Complete and Reproducible Workflow To Analyze iTRAQ/TMT Experiments.
Not available
S-EPMC6456869 | biostudies-literature
ProteomicsiTRAQ-TMT mass spectrometry in K562 cells
K562 cells were infected with none specific or Drosha CRISPR lenti-virus to silence the gene and subjected to iTRAQ-TMT mass spectrometry
ORGANISM(S): Homo Sapiens 
2020-12-23 | PXD023282 |
UnknownAnalysis of isobaric quantitative proteomic data using TMT-Integrator and FragPipe computational platform.
Not available
S-EPMC12154698 | biostudies-literature
ProteomicsLocal translation of mitochondrial proteins at the synapse
To systematically explore the dynamics of the synaptic proteome upon neuronal stimulation we employed a simple model system which is based on isolated synaptoeurosomes (SN). Synaptoneurosomes are preparations freshly obtained from the brain and enriched in synapses, containing both pre and postsyna...
ORGANISM(S): Mus musculus (Mouse) 
2020-08-12 | PXD012707 | Pride
Local translation Synaptosomes Quantitative proteomics 
ProteomicsLocal translation of mitochondrial proteins at the synapse, LFQ expriment
To systematically explore the dynamics of the synaptic proteome upon neuronal stimulation we employed a simple model system which is based on isolated synaptoeurosomes (SN). Synaptoneurosomes are preparations freshly obtained from the brain and enriched in synapses, containing both pre and postsyna...
ORGANISM(S): Mus musculus (Mouse) 
2020-08-12 | PXD012746 | Pride
Local translation Synaptosomes Quantitative proteomics 
ProteomicsImpaired iloprost-induced platelet inhibition and phosphoproteome changes in patients with confirmed pseudohypoparathyroidism type Ia, linked to genetic mutations in GNAS
Patients diagnosed with pseudohypoparathyroidism type Ia (PHP Ia) suffer from hormonal resistance. This is often accompanied by abnormal postural and facial features, brachydactyly and dermal calcifications, a condition classified as Albright hereditary osteodystrophy (AHO) syndrome. Typically, this...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD016534 | Pride
Human platelets Phosphoproteome Tmt Itraq Proteome Albright hereditary osteodystrophy 
ProteomicsMass spectrometry-based quantitative proteomics of whole cell lysate from breast cancer cell line MCF7 spiked with 57 protein standards
Here we present quantitative proteomics data used in the evaluation of quantitative accuracy. A human cell line, MCF7 was split into 9 aliquotes that were spiked with a dilution series of 57 protein standards of known amounts spanning 5 orders of magnitude. The protein extracts were trypsinized, and...
ORGANISM(S): Homo sapiens (Human) 
2013-12-03 | PXD000578 | Pride
Lc-ms Quantification Mcf7 Isoelectric focusing Itraq Tmt Precursor mixing 
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