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ProteomicsFLAG-affinity pulldown experiments with sll1214 and sll1874 from Synechocystis
Putative subunits of the Mg-protoporphyrin monomethylester oxidative cyclase from Synechocystis PCC 6803, sll1214 and sll1874 were expressed with N-terminal 3xFLAG-tags in separate transformants.. Solubilised thylakoid membranes from these 2 transformants were incubated with anti-FLAG agarose and af...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2015-08-05 | PXD000632 | Pride
Sll1214 Sll1874 Ycf54 Mg-protoporphyrin monomethylester oxidative cyclase Flag-tag Maxis uhr-tof Synechocystis pcc 6803 
ProteomicsAbsolute quantification of cellular levels of photosynthesis-related proteins in Synechocystis sp. PCC 6803
We quantified proteins in the model cyanobacterium Synechocystis sp. PCC 6803 given the general importance of cyanobacteria for global photosynthesis, for synthetic biology and biotechnology research, and their ancestral relationship to the chloroplasts of plants. Four mass spectrometry methods wer...
ORGANISM(S): Synechocystis sp. PCC 6803 substr. GT-I 
2023-01-04 | PXD035632 | Pride
Assembly factor Photosystem Electron transport Mass spectrometry Photosynthesis Synechocystis Cyanobacteria Chlorophyll 
ProteomicsComparative quantitative proteomic analysis of Chlamydomonas reinhardtii cells from wild-type and chimeric FNR-PSAF mutant cells
Photosynthetic linear electron transfer (LET) comprises a solar energy driven pathway tha directs electrons to the production of NADPH, via ferredoxin-NADP+ reductase (FNR), while generating a proton motive force (pmf) to drive ATP synthesis. Both NADPH and ATP are consumed for carbon fixation. Howe...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-05-07 | PXD052753 | Pride
Chloroplast Cyclic electron transfer Crispr-cas9 Photosystem (ps) Let Ferredoxin-nadp+ reductase Chlamydomonas reinhardtii Psi subunit psaf Linear electron transfer Cet 
ProteomicsThe membrane-intrinsic Zn-metalloproteinase FtsH4 does not associate with FtsH1, FtsH2 or FtsH3 but does interact with the uncharacterized protein Sll1106
FtsH Zn-metalloproteinases are multimeric, membrane-intrinsic and universally conserved in bacteria, chloroplasts and mitochondria. FtsHs function in protein turnover and processing: activities that are essential in maintaining cellular homeostasis. In phototrophic organisms, FtsHs are additionally ...
ORGANISM(S): Synechocystis sp. PCC 6803 substr. GT-I 
2023-01-04 | PXD033240 | Pride
Biogenesis Cyanobacteria Sll1106 Ftsh Synechocystis Flag-tag Photosystem Repair 
ProteomicsTwo unrelated 8-vinyl reductases ensure production of mature chlorophylls in Acaryochloris marina
The aim of this project is to identify, by bottom-up proteomic analysis, one or both representatives of two classes of 8-vinyl reductase in an Acaryochloris marina cell lysate. This enzyme is responsible for the conversion of the C8 vinyl group in the chlorophyll precursor Mg-divinyl-protochlorophyl...
ORGANISM(S): Acaryochloris marina (strain MBIC 11017) 
2020-05-26 | PXD003139 | Pride
Chlorophyll d biosynthesis Acaryochloris marina Photosynthesis 
ProteomicsComparative quantitative proteomic analysis of Arabidopsis thylakoid membranes from plants grown under low, moderate and high illumination. Effects of stn7 and tap38 mutations.
Plants grown under different illumination levels undergo a long-term acclimation response (LTR) resulting in differences in their leaf morphology and chloroplast protein composition to maximise their growth. At the proteomic level, LTR involves modulating the stoichiometry of photosynthetic complexe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-03-30 | PXD037358 | Pride
Arabidopsis thaliana Tap38 Stn7 Light harvesting complex Photosystem Thylakoid Electron transfer. Light acclimation 
ProteomicsProteomic comparison of HliD and Ycf39 protein abundances in FLAG-tagged chlorophyll synthase co-immunoprecipitation assays of control and CrtR/CruF (carotenoid biosynthesis) knockout strains of Synechocystis sp. PCC 6803.
Chlorophyll a synthase (ChlG) is the enzyme responsible for attaching either a geranygeranyl or phytyl group to the chlorophyllide macrocycle during chlorophyll biosynthesis. Previous co-immunoprecipitation assays using FLAG-tagged ChlG identified the high light inducible protein HliD and the photos...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2022-08-18 | PXD020471 | Pride
Zeaxanthin Chlorophyll a synthase Flag-tag Hlid Myxoxanthophyll Synechocystis sp. pcc 6803 Ycf39 Crtr Cruf. 
ProteomicsFunctions of the FtsH1/3 complex in the nutrient stress response of Synechocystis
FtsH zinc metalloproteinases are membrane-intrinsic and occur in bacteria, chloroplasts, and mitochondria. They are involved in diverse cellular processes including protein quality control and regulation. The cyanobacterium Synechocystis possesses four FtsH homologs, designated FtsH1-4 which functio...
ORGANISM(S): Synechocystis sp. (strain PCC 6803 / Kazusa) 
2021-08-13 | PXD013401 | Pride
Nutrient stress Ftsh Synechocystis Transcription factors 
ProteomicsStructural characterization of the reaction center-light harvesting 1-PufXY complex from Rhodobacter sphaeroides
Photosynthesis in purple bacteria, represented by Rhodobacter sphaeroides as a long-established model organism, utilizes a protein-cofactor complex to capture solar energy. This complex is embedded in specialized spherical membrane structures and comprises a central reaction center (RC) encircled by...
ORGANISM(S): Rhodobacter sphaeroides 2.4.1 
2022-02-15 | PXD028048 | Pride
Lc-ms/ms Rhodobacter sphaeroides Purple bacteria Photosynthesis Reaction center light-harvesting-1-pufxy complex 
ProteomicsProteomic analysis of the reaction center-light harvesting 1 complex from Rhodobacter sphaeroides, identifying a previously uncharacterized protein component encoded by a newly-identified ORF.
Photosynthesis in purple bacteria, represented by Rhodobacter sphaeroides as a long-established model organism, utilizes a protein-cofactor complex to capture solar energy. This complex is embedded in specialized spherical membrane structures and comprises a central reaction center (RC) encircled by...
ORGANISM(S): Rhodobacter sphaeroides 2.4.1 
2022-02-16 | PXD028553 | Pride
Lc-ms/ms Rhodobacter sphaeroides Purple bacteria Photosynthesis Dimerization Reaction center light-harvesting-1- complex 
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