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ProteomicsDefining, Comparing, and Improving iTRAQ Quantification in Mass Spectrometry Proteomics Data
The purpose of this study was to generate a basis for the decision of what protein quantities are reliable and find a way for accurate and precise protein quantification. To investigate this we have used thousands of peptide measurements to estimate variance and bias for quantification by iTRAQ (iso...
ORGANISM(S): Homo sapiens (Human) 
2013-08-16 | PXD000418 | Pride
Ipg-ief Itraq Lc-ms/ms 
ProteomicsRetinoic acid receptor alpha is associated with tamoxifen resistance in breast cancer
About one-third of oestrogen receptor alpha- positive breast cancer patients treated with tamoxifen relapse. Here we identify the nuclear receptor retinoic acid receptor alpha as a marker of tamoxifen resistance. Using quantitative mass spectrometry-based proteomics, we show that retinoic acid recep...
ORGANISM(S): Homo sapiens (Human) 
2013-07-24 | PXD000281 | Pride
Human Breast cancer Tamoxifen Ipg-ief Nlc-ms Drug resistance Subcellular fractionation 
ProteomicsHs_Pleural_Effusion_8plex
Human pleural effusion LC-MSMS, 8plex
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005065 | PeptideAtlas
ProteomicsCharacterization of the PAK4 interactome by quantitative mass spectrometry
p21-activated kinase 4 (PAK4) is a serine/threonine kinase critical during development and with a proposed role in cancer and related cellular processes, including cell proliferation, survival and migration. However, while a limited number of PAK4-interacting proteins have been identified, the PAK4 ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-27 | PXD004086 | Pride
Ms/ms Pull-down Protein-protein interactions Pak4 
Proteomics,MultiomicsHiRIEFII proteomics and proteogenomics of A431 cells and five histologically normal tissues
High Resolution Isoelectric Focusing (HiRIEF) LC-MS was used to analyze samples from the human A431 cell line and also samples of histologically normal tissues. The high analytical depth afforded by HiRIEF permitted proteogenomics on these two sample sets (A431 cells and normal tissues). The A431 d...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-10-04 | MSV000083000 | MassIVE
ProteomicsProteome characterisation of Malassezia sympodialis microvesicles
The data sets contain an iTRAQ 8plex set for identification of proteins enriched in microvesicles (4 replicates) compared to whole cells (4 replicates).
ORGANISM(S): Malassezia Sympodialis 
2018-09-11 | PXD009113 | JPOST Repository
ProteomicsE.Coli spike in to human cell line MCF-7
Spike in of 3 different amounts of E.Coli to human cell line MCF-7 with TMT10plex quantification.
ORGANISM(S): Homo Sapiens (human) 
2020-03-28 | PXD013277 | JPOST Repository
Proteomics,MultiomicsHiRIEF deep proteomics of A431 human vulvar cancer cell line and N2A mouse neuroblastoma cell line
Here we present high resolution data from two cancer cell lines. A human cell line, A431 was treated with a drug at different time points and then fractionated into its different subcellular compartments (nuclear, organellar, soluble,whole cell). Protein extracts were trypsinized, peptides separated...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2016-07-20 | MSV000079945 | MassIVE
ProteomicsProteome profiling of the blood brain barrier in mice
To characterize the vascular proteome of the brain, proteins were extracted from the brain microvascular fragments isolated from adult mice using CD31-coated magnetic beads. The proteins were enzymatically digested and subjected to mass spectrometric analysis
ORGANISM(S): Mus Musculus (mouse) 
2026-08-07 | PXD071189 | JPOST Repository
ProteomicsTime-course proteomics analysis of human regulatory T cell (Treg) differentiation
Human naïve CD4+ T cells (CD4+ CD45RA+ CD25- CD45RO- CD8- CD14- CD15- CD16- CD19- CD34- CD36- CD56- CD123- TCRγ/δ- HLA-DR- and CD235a-) were magnetically negatively isolated from peripheral blood. Cells were stimulated with anti-CD3/anti-CD28 antibodies plus IL-2, and samples were taken at 6h, 24h, ...
ORGANISM(S): Homo Sapiens (human) 
2018-04-10 | PXD005703 | JPOST Repository
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