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ProteomicsProteomic profiling of glioblastoma stem cells, human astrocyte, and T98G glioblastoma cell line
With HiRIEF LC-MS/MS shotgun proteomics, we analysed 6 patient-derived glioblastoma stem cells (BT stem cells) and compared them to an astrocyte line (CliniSciences, Guidonia Montecelio, Italy) and a more differentiated glioblastoma cell line (T98G). Each of the 8 cell line sample was run in triplic...
ORGANISM(S): Homo sapiens (Human) 
2022-12-23 | PXD027335 | Pride
Glioblastoma Stem cells Hirief Brain cancer T98g Cell line Lc-ms/ms Astrocyte 
ProteomicsMembrane enriched proteome of transfected SW480 cells
This project used membrane-enriched proteomics to compare the membrane proteome of SW480 human colorectal cancer subclones that had been transfected with the full length coding sequence of the beta-6 integrin subunit under an overexpression promoter (SW480OE) against SW480 cells that had been transf...
ORGANISM(S): Homo sapiens (Human) 
2013-05-30 | PXD000230 | Pride
Membrane proteomics Colorectal cancer Integrin Sw480 Lc-ms/ms 
UnknownComparison of in-gel protein separation techniques commonly used for fractionation in mass spectrometry-based proteomic profiling.
Not available
S-EPMC4234072 | biostudies-literature
ProteomicsDefining, Comparing, and Improving iTRAQ Quantification in Mass Spectrometry Proteomics Data
The purpose of this study was to generate a basis for the decision of what protein quantities are reliable and find a way for accurate and precise protein quantification. To investigate this we have used thousands of peptide measurements to estimate variance and bias for quantification by iTRAQ (iso...
ORGANISM(S): Homo sapiens (Human) 
2013-08-16 | PXD000418 | Pride
Ipg-ief Itraq Lc-ms/ms 
ProteomicsProteome profiling of 2 dystrophic mouse models
Proteome profiling of tibialis anterior muscle from C57BL/6 mice and two dystrophin strains (mdx52 and mdx) at 8, 16, and 80 weeks in triplicates. TMT10 plex quantification and IPG-IEF pH 3.7-4.9 fractionation of peptides before nanoLC-MS/MS analysis on an Q-exactive instrument.
ORGANISM(S): Mus Musculus (mouse) 
2020-10-01 | PXD017169 | JPOST Repository
UnknownImmobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis.
Not available
S-EPMC3170271 | biostudies-literature
UnknownOptimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach.
Not available
S-EPMC517948 | biostudies-literature
UnknownProteomics profiling identify CAPS as a potential predictive marker of tamoxifen resistance in estrogen receptor positive breast cancer.
Not available
S-EPMC4389343 | biostudies-literature
ProteomicsSubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
We have devised a simple and robust LC-MS/MS compatible protocol for subcellular protein localization studies. Here, we combine it with in depth proteome profiling and utilize the resulting dataset to predict the subcellular localization of more than 12000 proteins in 5 human cancer cell lines. The ...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD006895 | Pride
A431 Mcf7 U251 Human Cancer Hcc827 Subcellular protein localization Hirief Subcellular Nci-h322 Lc-ms. 
UnknownComparative proteomics analysis of differential proteins in respond to doxorubicin resistance in myelogenous leukemia cell lines.
Not available
S-EPMC4307195 | biostudies-literature
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