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TranscriptomicsA comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus
Salt loading (SL) and water deprivation (WD) are experimental challenges that are often used to study the osmotic circuitry of the brain. Central to this circuit is the supraoptic nucleus (SON) of the hypothalamus, which is responsible for the biosynthesis of the hormones, vasopressin (AVP) and oxyt...
ORGANISM(S): Rattus norvegicus 
2015-02-05 | E-GEOD-65663 | biostudies-arrayexpress
TranscriptomicsMetabolic Reprogramming During Purine Stress in the Protozoan Pathogen Leishmania donovani
The ability of Leishmania to survive in their insect or mammalian host is dependent upon an ability to sense and adapt to changes in the microenvironment. However, little is known about the molecular mechanisms underlying the parasite response to environmental changes, such as nutrient availability....
ORGANISM(S): Leishmania donovani 
2014-02-01 | E-GEOD-48394 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse vehicle and sAPPalpha-treated hippocampal slice cultures
Gene expression changes induced by alpha-secretase cleaved amyloid precursor protein (sAPPalpha) in organotypic hippocampal slice cultures of male, postnatal day 15 mice (C57B6/SJL). Hippocampal slice cultures were treated with phosphate buffered saline (GSM26700, GSM26701, GSM26702) or 1 nM sAPPal...
ORGANISM(S): Mus musculus 
2007-10-10 | E-GEOD-1555 | biostudies-arrayexpress
MetabolomicsEvidence for quorum sensing and differential metabolite production by Ruegeria pomeroyi in response to DMSP
Microbial alteration and remineralization of organic matter in the ocean play essential roles in the carbon cycle. The impact of individual compounds as either growth substrates or infochemicals is still poorly constrained in models, particularly for organisms with relatively versatile genomes. Here...
2015-01-29 | MTBLS157 | MetaboLights
TranscriptomicsHalobacterium NRC-1 Diurnal experiment Light 2
The wild type strain of Halobacterium salinarium strain NRC-1 was used for the diel growth experiments. For experiment, cultures were started at an OD >> 0.001. Cultures were grown anaerobically at a temperature of 38° C under light:dark cycles (12:12 hrs) for 120 hours then grown under constant ...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7712 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and bop perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of bop was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7717 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and sop2 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of sop2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7719 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and sop2 perturbation, experiment 2
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of sop2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clea...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7736 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 General transcription factor perturbation
Halobacterium cells, specified general transcription factor knock-out strains or plasmid expressed 6-His tagged general transcription factor strains, were grown to mid-log phase. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-27 | E-GEOD-7713 | biostudies-arrayexpress
TranscriptomicsHalobacterium NRC-1 oxygen, light and ura3 perturbation
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illu...
ORGANISM(S): Halobacterium sp. NRC-1 
2010-05-26 | E-GEOD-7715 | biostudies-arrayexpress
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