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ProteomicsCharacterisation of the Active Kinome of Leishmania
Background: Leishmania parasites cause neglected tropical diseases such as cutaneous and visceral leishmaniasis, which have limited treatment options and rising drug resistance. Protein kinases are pivotal in Leishmania biology and attractive drug targets, but their functional status in the parasite...
ORGANISM(S): Leishmania mexicana 
2025-12-18 | PXD066933 | Pride
Parasite Drug target Leishmania Chemoproteomics Neglected tropical disease Activity-based protein profiling Protein kinase Kinome 
ProteomicsDruggable Serine Proteases of Leishmania by Activity‐Based Protein Profiling
Leishmaniasis are a group of diseases caused by parasitic protozoa of the genus Leishmania. Current treatments are limited by difficult administration, high cost, poor efficacy, toxicity, and growing resistance. New agents, with new mechanisms of action, are urgently needed to treat the disease. Alt...
ORGANISM(S): Leishmania mexicana 
2022-07-04 | PXD033616 | Pride
Activity-based protein profiling Leishmania Serine protease 
ProteomicsQuantitative proteomics profiling of NSPs in Leishmania mexicana parasites during starvation
Using a combination of BONCAT technique and iTRAQ 4-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the h...
ORGANISM(S): Trypanosoma brucei 
2019-12-03 | PXD014580 | Pride
Boncat Proteomics Traslation Itraq Leishmania mexicana 
ProteomicsQuantitative proteomics of Hsp90 inhibition in Leishmania mexicana
Using a combination of mass spectrometry-based quantitative proteomics, chemical and metabolic labelling, we provide the first protein-level evidence that the Hsp90 inhibition affects the expression of hundreds of proteins in Leishmania spp. Our study defines the Leishmania parasite’s response to Hs...
ORGANISM(S): Leishmania mexicana 
2021-04-23 | PXD024764 | Pride
Leishmania Quantitative proteomics 
ProteomicsQuantitative chemical proteomics of Leishmania mexicana
We report the first systematic study of the effect of Hsp90 inhibition on the global protein synthesis in Leishmania parasites using an integrated chemical biology approach. Heat shock protein 90 (Hsp90) is a conserved molecular chaperone responsible for the folding of newly synthesised proteins. It...
ORGANISM(S): Leishmania mexicana mexicana 
2021-04-23 | PXD022708 | Pride
Leishmania Itraq 
ProteomicsMolecular markers of adaptive responses in Toxoplasma gondii to Pyrimethamine treatment
In this study, we employed bio-orthogonal non-canonical amino acid tagging (BONCAT) combined with iTRAQ-based quantitative proteomics to investigate the effects of pyrimethamine (PM) on newly synthesised proteins (NSPs) in Toxoplasma gondii. Parasites were treated with varying concentrations of PM, ...
ORGANISM(S): Toxoplasma gondii 
2025-05-07 | PXD054994 | Pride
Boncat Pyrimethamine Toxoplasma gondii Quantitative proteomics Itraq 
ProteomicsGlobal phosphoproteome of Leishmania mexicana developmental stages
Using a combination of phosphoproteome enrichment and tandem mass tag (TMT) labelling-based quantitative proteomic mass spectrometry (MS), we robustly identified and quantified 1,833 phosphorylated proteins across three life cycle stages of Leishmania mexicana (L. mexicana) parasite. Protein kinase ...
ORGANISM(S): Leishmania mexicana MHOM/GT/2001/U1103 
2023-10-04 | PXD043002 | Pride
Phosphoproteome Tmt labelling Leishmania 
ProteomicsTranscriptome-wide identification of coding and noncoding RNA-binding proteins defines the extended RNA interactome of Leishmania mexicana
Non-poly(A) RNA molecules including noncoding RNAs (ncRNAs) comprise the major portion of the total transcribed molecules in the cell. In addition to the mRNAs the ncRNAs also function as ribonucleoprotein particles (RNPs) and carry out biological functions including synthesis of new proteins, RNA p...
ORGANISM(S): Leishmania mexicana MHOM/GT/2001/U1103 
2022-01-14 | PXD028092 | Pride
Leishmania Rna-binding proteins 
ProteomicsStarvation-specific NSPs in Leishmania mexicana parasites by quantitative proteomics
Using a combination of BONCAT technique and iTRAQ 3-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the h...
ORGANISM(S): Trypanosoma brucei 
2019-12-04 | PXD015827 | Pride
Newly synthesised proteins Starvation Boncat Leishmania Itraq 
ProteomicsLarge-scale Profiling of ADP-Ribosylome by Chemical Proteomics
We have developed a dual metabolic incorporation of clickable NAD precursor compounds in live mammalian cells and in combination with the tandem mass tag (TMT) isobaric mass spectrometry, we have quantified the responses of thousands of endogenous proteins to clinical PARP inhibitors Olaparib and Ru...
ORGANISM(S): Homo sapiens (Human) 
2019-05-01 | PXD011631 | Pride
Tmt Mda-mb-231 Nad 
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