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ProteomicsInhibition of L-type amino acid transporter 1 induces disruption of cancer amino acid homeostasis with altered cellular signals
This study characterized the changes of phosphorylation signaling cascades induced by LAT1 inhibition in three cell lines of biliary tract cancer treated with LAT1 specific inhibitor JPH203 for 15 and 30 min. Immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometr...
ORGANISM(S): Homo Sapiens (human) 
2023-05-24 | PXD034276 | JPOST Repository
ProteomicsIntegrated proteomics and phosphoproteomics analyses reveal the broad and complex influence by inhibition of L-type amino acid transporter-1 in biliary tract carcinoma cells
To characterize the changes of phosphorylation signaling cascades and protein expressions induced by LAT1 inhibition in 4 cell lines of biliary tract cancer treated with LAT1 specific inhibitor JPH203, immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometry with ...
ORGANISM(S): Homo Sapiens (human) 
2020-12-25 | PXD019458 | JPOST Repository
ProteomicsTMT-10plex analysis of endogenous proteins expressed in HEK-293 cells as a recombinant transporter assay system
Tandem-mass-tags (TMT)-10plex proteomics characterized endogenous proteins expressed in HEK-293 cells transiently transfected with control vector or transporter cDNA. Proteomic profiles between mock (n=3), naïve (n=3), and HEK-293 cells transfected with MATE1 cDNA (1, 10, 30, 100 ng) were compared.
ORGANISM(S): Homo Sapiens (human) 
2022-07-07 | PXD030022 | JPOST Repository
ProteomicsLauroylation-assisted workflow for both PICS and N-terminomics
(1) Lauroylation-assisted workflow for PICS using E. coli proteome-derived tryptic peptide libraries which were blocked (succinylation or dimethylation). (2) Lauroylation-assisted N-terminomics to profile protein N-termini, ORF and non-ORF protein N-termini, using etoposide-treated cholangiocarcinom...
ORGANISM(S): Homo Sapiens (human) Escherichia Coli 
2026-05-01 | PXD067596 | JPOST Repository
ProteomicsLauroylation-assisted workflow for N-terminomics with three biological replicates
Lauroylation-assisted N-terminomics for profiling protein N-termini, including ORF and non-ORF protein N-termini, using three biological replicates of etoposide-treated cholangiocarcinoma cells and exploiting negative selection.
ORGANISM(S): Homo Sapiens (human) 
2026-05-01 | PXD073552 | JPOST Repository
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsMembrane proteome of renal BBMVs from the mouse model
In order to demonstrate the efficacy of the membrane enrichment method by Urea Wash in the animal model. Kidneys were dissected from 8 weeks old male mice of inbred strain C57BL/6J and flash-frozen in liquid nitrogen followed by storage at -80 °C. Brush border membrane vesicles (BBMV) were prepared...
ORGANISM(S): Mus Musculus (mouse) 
2022-04-28 | PXD027474 | JPOST Repository
ProteomicsMembrane proteome of HEK293T cells
We developed a simple and effective method for “in-depth” membrane proteomics. We optimize the sample preparation for the maximum numbers of the identified membrane proteins using a human cell line, HEK293T, as a model. From this study, we demystified the merits of Urea wash methods for membrane enr...
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027473 | JPOST Repository
ProteomicsProteomic analysis of cochlear perilymph in a mouse model of Alzheimer's disease
This study referred to the changes in protein abundance of App(NL-GF/NL-GF) and App(wt/wt) mice perilymph. Liquid chromatography-tandem mass spectrometry with MS2-based quantification of tandem mass tag was utilized.
ORGANISM(S): Mus Musculus (mouse) 
2024-04-15 | PXD046317 | JPOST Repository
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