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ProteomicsA nanoLC-MS/MS system using a nonporous reverse phase column for ultrasensitive proteome analysis
The design of proteome projects is often restricted by the available amounts of sample, and thus there has been much effort to date to improve the sensitivity of proteome analyses. We have developed a new ultrasensitive nanoLC-MS system for the proteome analysis of microscale samples using a nonporo...
ORGANISM(S): Homo Sapiens (human) 
2018-09-24 | PXD011168 | JPOST Repository
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of coIP samples.
ORGANISM(S): Homo Sapiens (human) 
2024-03-06 | PXD048100 | JPOST Repository
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of 100ng of HEK293T proteins, coIP samples, and serum extracellular vesicles, and further challenged label-free single-cell proteomics o...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2024-03-06 | PXD044605 | JPOST Repository
ProteomicsIn-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.
We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each cultur...
ORGANISM(S): Homo Sapiens (human) 
2022-02-22 | PXD024272 | JPOST Repository
ProteomicsProteome analysis by simple and stable nanoESI spray system
We developed InSpIon system capable of stable nanoESI spray. Here, K562 cell digests were measured by DDA-MS and DIA-MS with the typical open nanoESI spray system (Open system) and the spray system inserted spray tip head into ion transfer tube (Insertion-ITT system), and the InSpIon system.
ORGANISM(S): Homo Sapiens (human) 
2023-04-05 | PXD039557 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by STL, LEL and WGA
Serum proteins enriched by solanum tuberosum lectin (STL), lycopersicon esculentum lectin (LEL) and wheat germ agglutinin (WGA) were analyzed by LC-DIA-MS/MS using Q-Exactive HF-X (ThermoFisher Scientific).
ORGANISM(S): Homo Sapiens (human) 
2024-02-24 | PXD048327 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by lectins
Serum proteins enriched by 37 different lectins were analyzed by LC-MS/MS. It was found that the enrichment process with Solanum tuberosum lectin (STL) and Lycopersicon esculentum lectin (LEL) detected many trace proteins in the serum. In addition, combinations of lectins were examined, and the comb...
ORGANISM(S): Homo Sapiens (human) Gallus Gallus Rattus Norvegicus Ovis Aries Cellular Organisms Capra Hircus Bos Taurus Mus Musculus (mouse) 
2024-02-23 | PXD047848 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by MOA and PSL1a
We conducted serum protein enrichment using Marasmius oreades agglutinin (MOA) and Polyporus squamosus lectin (PSL1a), followed by analysis using HF-X.
ORGANISM(S): Homo Sapiens (human) 
2025-04-30 | PXD051875 | JPOST Repository
ProteomicsProteomic Profiling of HEK293-F cell and human peripheral blood neutrophil proteins extracted by conventional direct extraction and phenol-guanidinium isothiocyanate (P/GTC)-based extraction methods
To evaluate the performance of the phenol-guanidinium isothiocyanate (P/GTC)-based extraction method in proteomic analysis, we performed proteome analysis from HEK293-F cell and human peripheral blood neutrophil proteins extracted by the P/GTC-based extraction and conventional direct extraction meth...
ORGANISM(S): Homo Sapiens (human) 
2019-10-28 | PXD011487 | JPOST Repository
ProteomicsValidation of Thin-diaPASEF for maximizing proteome coverage
We optimized the parameters of CCS range, polygon scan region, ramp time, and isolation window width to achieve high-depth proteome analysis using the timsTOF HT. As a result, we developed Thin-diaPASEF, which was optimized with a CCS range of 0.7–1.3, a ramp time of 150 ms, an isolation window widt...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2025-08-10 | PXD061613 | JPOST Repository
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