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ProteomicsProteome and phosphoproteome analysis of autophagy deficient ATG5flox:CamKII-Cre and ATG5flox:VGAT-Cre conditional knockout mice
The experiment was performed to find selective targets of autophagy in different subtypes of neurons. Therefore, brain tissue of conditional knockout mice lacking autophagy protein ATG5 in inhibitory or excitatory neurons was used. Cortices (ATG5flox:CamKII-Cre) / striata (ATG5flox:VGAT-Cre) of mice...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-15 | PXD029435 | Pride
Atg5 Neurons Autophagy Brain 
ProteomicsDetection of putative PKA target phosphorylation sites in Atg5flox:CamKII-Cre wildtype and knockout mice brain tissue
The experiment was performed to identify PKA phosphorylation substrates in wildtype and autophagy-deficient brains. Therefore, hippocampus and cortex of wildtype and conditional knockout mice were isolated and sliced. The brain slices were incubated with DMSO or Forskolin, a cAMP elevating agent, to...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-15 | PXD031106 | Pride
Forskolin Autophagy-deficiency Brain slices Pka signalling 
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