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ProteomicsLoss of H3K9 tri-methylation alters mitotic chromosome compaction and transcription factor retention during mitosis
Recent studies have shown that repressive chromatin machinery, including DNA methyltransferases (DNMTs) and Polycomb Repressor Complexes (PRCs), bind to chromosomes throughout mitosis and their depletion results in increased chromosome size. Enzymes that catalyse H3K9 methylation, such as Suv39h1, S...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-12 | PXD030771 | Pride
Chromatin  mitotic chromosomes  h3k9me3  suv39h1  suv39h2  ezh2  esrrb 
ProteomicsGrowth Rate Dependent and Nutrient-specific Gene Expression Resource Allocation in Fission Yeast
Cellular resources are limited and their relative allocation to gene expression programmes determines physiological states and global properties such as the growth rate. Quantitative studies using various growth conditions have singled out growth rate as a major physiological variable explaining rel...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2022-02-24 | PXD027835 | Pride
Resource allocation Schizosaccharomyces pombe Fission yeast Gene expression Transcriptomics Proteomics 
ProteomicsAutophagosome Proteomics of HEK293 cells
Pan-caspase inhibitor Z-VAD-fmk acts as an inhibitor of peptide:N-glycanase (NGLY1); an endoglycosidase which cleaves N-linked glycans from glycoproteins exported from the endoplasmic reticulum during ER-associated degradation (ERAD). Pharmacological N-glycanase inhibition by Z-VAD-fmk or siRNA knoc...
ORGANISM(S): Homo sapiens (Human) 
2022-01-12 | PXD020367 | Pride
Autophagy  autophagosome  ngly1  n-glycanase  z-vad-fmk  off-target 
ProteomicsIdentification of C2CD4A and C2CD4B binding partners in MIN6 cells
Variants close to the VPS13C/C2CD4A/C2CD4B locus are associated with altered risk of type 2 diabetes in genome-wide association studies. Whilst previous functional work has suggested roles for VPS13C and C2CD4A in disease development, none has explored the role of C2CD4B. Here, we show that systemic...
ORGANISM(S): Mus musculus (Mouse) 
2020-11-16 | PXD021597 | Pride
C2cd4a  c2cd4b  interaction proteomics 
ProteomicsProteomics analysis of rat primary myocyte and neuron-myocyte co-cultures
Alterations in autonomic function are known to occur in cardiac conditions including sudden cardiac death. Cardiac stimulation via sympathetic neurons can potentially trigger arrhythmias. Dissecting direct neural-cardiac interactions at the cellular level is technically challenging and understudied ...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-07-21 | PXD019908 | Pride
Cardiomyocyte network dynamics  cardiac arrhythmias  myocyte  co-culture 
ProteomicsNovel protein interaction network of human calcitonin receptor-like receptor revealed by label-free quantitative proteomics
Background : The G protein-coupled receptor (GPCR) calcitonin receptor-like receptor (CLR) is implicated in cardiovascular and skin diseases, migraine and cancer. Beyond its agonists, receptor activity-modifying proteins and receptor component protein, interacting partners of this GPCR (‘CLR interac...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD032156 | Pride
Skin. Endothelial cell Human Lymphatic Proteome 
ProteomicsAnalysis of factors bound to native metaphase ESC chromosomes following purification by flow cytometry
Stem cells are able to self-renew and also generate differentiated progeny. How gene expression patterns are transmitted through mitosis remains unresolved. Conventional studies have relied upon rigorous cell-cycle-stage synchronisation to find factors in mitotic lysates that could ‘bookmark’ the ge...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-15 | PXD015251 | Pride
Mitosis Stem cells Chromosomes Chromatin 
ProteomicsCaspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology
Contactin-associated protein-like 2 (Caspr2) is a neurexin-like protein that has been associated with numerous neurological conditions. However, the mechanisms underlying Caspr2 function in the central nervous system remain incompletely understood. Here, we report on a functional role for Caspr2 in ...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-13 | PXD018972 | Pride
5-trisphosphate receptor type 1  cerebellum  purkinje cell 4 Caspr2  itpr  inositol 1 
ProteomicsCritical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin
The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1, however, how UHRF1 deposits ubiquitin onto the histone is unknown. H...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-11-04 | PXD011510 | Pride
Ubiquitylation Ubiquitin-like fold Uhrf1 Histone modification Dna methylation Chromatin 
ProteomicsPhosphoproteomics of DNA repair in Saccharomyces cerevisisae
The role of Rad53 in response to a DNA lesion is central for the accurate orchestration of the DNA damage response. Rad53 activation relies on its phosphorylation by the Mec1 kinase and its own autophosphorylation in a manner dependent on the adaptor Rad9. While the mechanism behind Rad53 phosphoryl...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-09-12 | PXD013782 | Pride
Dna repair/double-strand break  homologous recombination  pp4  resection  phosphoproteomics 
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