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TranscriptomicsGene expression profiles of uhrf1 mutant zebrafish
UHRF1 (Ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication, is essential for maintaining DNA methylation patterns during cell division and is suggested to direct additional repressive epigenetic marks. Uhrf1 mutation in zebrafish results...
ORGANISM(S): Danio rerio 
2015-02-09 | E-GEOD-55339 | biostudies-arrayexpress
ProteomicsIdentificaiton of novel acetylation sites in UHRF1 mediated by acetyltransferase MOF
The multi-domain protein UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 for DNA methylation maintenance during DNA replication. Here, we show that MOF (Males absent On the First) is an acetyltransferase of UHRF1 to acetylate UHRF1 at Lys670 in the pre-RING linker re...
ORGANISM(S): Homo sapiens (Human) 
2024-03-06 | PXD040426 | Pride
Acetylation  uhrf1  mof 
ProteomicsUhrf1 loss disrupts CTCF-associated chromatin organization during early mouse embryogenesis
UHRF1 is a chromatin-binding protein essential for maintaining DNA methylation and histone modification states, yet its integrated role in vivo remains incompletely understood. To define its function, we generated conditional Uhrf1 knockout embryonic stem cells (ESCs) and embryos. Uhrf1⁻/⁻ ESCs exhi...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-17 | PXD084341 | Pride
Chromatin organization Uhrf1 Dna methylation Embryonic development 
TranscriptomicsExpression data from HeLa cell lines stably expressing shRNA targeting ctrl or UHRF1
We used microarrays to explore the expression profile from cells expressing wild type and UHRF1 S674A mutant. HeLa cells expressing UHRF1 WT and S674A mutant showed similar gene expression pattern without significant affecting the transcription of DNA repari genes. UHRF1 was depleted in HeLa cells...
ORGANISM(S): Homo sapiens 
2016-01-05 | E-GEOD-73751 | biostudies-arrayexpress
TranscriptomicsPHD finger recognition of unmodified histone H3R2 links UHRF1 to regulation of euchromatic gene expression
Histone methylation occurs on both lysine and arginine residues and its dynamic regulation plays a critical role in chromatin biology. Here we identify the UHRF1 PHD domain (PHDUHRF1), an important regulator of DNA CpG methylation, as an unanticipated histone H3 unmodified arginine 2 (H3R2)-recognit...
ORGANISM(S): Homo sapiens 
2011-07-07 | E-GEOD-30478 | biostudies-arrayexpress
ProteomicsUbiquitome analysis of UHRF1 and UHRF2-deficient mESCs
The E3 ligase UHRF1 is an essential epigenetic cofactor for DNMT1 dependent maintenance DNA methylation, which provides a binding platform for DNMT1 by both cooperative binding of histones and hemi-methylated DNA as well as by ubiquitinating histone H3. Here, we conduct a comprehensive screen to ide...
ORGANISM(S): Mus musculus (Mouse) 
2017-10-24 | PXD006593 | Pride
Ubiquitination Paf Mouse embryonic stem cells Uhrf 
ProteomicsCritical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin
The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1, however, how UHRF1 deposits ubiquitin onto the histone is unknown. H...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-11-04 | PXD011510 | Pride
Ubiquitylation Ubiquitin-like fold Uhrf1 Histone modification Dna methylation Chromatin 
ProteomicsHemi-methylated DNA regulates DNA methylation inheritance through allosteric control of H3 ubiquitylation by UHRF1
DNA methylation is a heritable chromatin modification essential to mammalian development that functions with histone post-translational modifications to regulate chromatin structure and gene expression programs. The epigenetic inheritance of DNA methylation requires the combined actions of DNMT1 and...
ORGANISM(S): Homo sapiens (Human) 
2016-08-25 | PXD003983 | Pride
Uhrf1 Ring e3 ligase Epigenetics Histone post-translational modifications Dna methylation Ubiquitin Histone h3 ubiquitylation 
ProteomicsIdentification of ubiquitination targets of UHRF1
The reversible attachment of ubiquitin governs the interaction, activity and degradation of proteins whereby the type and target of this conjugation determine the biological response. The investigation of this complex and multi-faceted protein ubiquitination mostly relies on painstaking biochemical ...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-30 | PXD034335 | Pride
Posttranslational modifications (pmt) Ubiquitination Protein degradation Dna methylation Heterochromatin binding protein (hp1) Uhfr1 
GenomicsBisulfite seq of UHRF1 KO A549 cells
Bisulfite seq of UHRF1 KO A549 cells
PRJNA1119457 | ENA
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