Sort   by:  
 Page size 
ProteomicsRecombinant Protein Spectral Library (rPSL) DIA-MS Method Improves Identification and Quantification of Low-Abundance Cancer-Associated and Kynurenine Pathway Proteins
Data-independent acquisition mass spectrometry (DIA-MS) is a powerful tool for quantitative proteomics, but a well-constructed reference spectral library is crucial to optimize DIA analysis, particularly for low-abundance proteins. In this study, we evaluate the efficacy of a recombinant protein spe...
ORGANISM(S): Homo sapiens (Human) 
2025-05-15 | PXD061696 | Pride
Snap frozen tissues Rpsl Dia-ms Cancer- associated proteins Cell lysates Kynurenine pathway proteins Recombinant protein spectral library 
MetabolomicsA Metabolomic Approach for Predicting Diurnal Changes in Cortisol

INTRODUCTION: The dysregulation of cortisol secretion has been associated with a number of mental health and mood disorders. However, diagnostics for mental health and mood disorders are behavioral and lack biological contexts.

OBJECTIVES: The goal of this ...

2021-09-23 | MTBLS1206 | MetaboLights
UnknownGC-MS analysis of phytoconstituents from Amomum nilgiricum and molecular docking interactions of bioactive serverogenin acetate with target proteins.
Not available
S-EPMC7532471 | biostudies-literature
ProteomicsMapping the complexity of ME/CFS: Evidence for abnormal energy metabolism, altered immune profile and vascular dysfunction
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disorder with no known underlying mechanisms, diagnostic tools, or treatments. Multiple areas of dysfunction have been extensively studied, but rarely examined together. We recruited age- and sex-matched ME/CFS patients and he...
ORGANISM(S): Homo sapiens (Human) 
2025-11-14 | PXD068504 | Pride
Metabolomics Immune dysfunction Energy metabolism Myalgic encephalomyelitis/chronic fatigue syndrome 
ProteomicsReduced structural rigidity of MDMX protein enhances binding to TP53 mRNA
The two murine double minute (MDM) family members, MDM2 and MDMX are a well-established negative regulator of p53 activity. Under DNA damage conditions, MDM2 and MDMX are phosphorylated near their RING domains (serine 395 at MDM2 and serine 403 at MDMX) and switch to act as p53 positive regulators. ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD060074 | Pride
Human Mdmx-mdm2 interaction P53 mrna stabilization Ring domain Hdx-ms mapping Lc-ms/ms 
ProteomicsIdentification of RSRC2 interactome through Immunoprecipitation (IP) coupled with LC-MS/MS-based protein analysis
We immunoprecipitate (IP) RSRC2 from HCT116 cells in the presence or absence of RNase A/T1 mix to identify RNA-mediated and RNA-independent RSRC2 protein-protein interaction networks. Four biological replicates were performed for each IP-MS in cells +/- RNase A/T1 Mix (EN0551, ThermoFisher) using Ig...
ORGANISM(S): Homo sapiens (Human) 
2026-03-12 | PXD059507 | Pride
Label-free quantification  interactome analysis  rsrc2  ip-ms 
ProteomicsRNA antisense oligonucleotide pulldown coupled with mass spectrometry (MS) to identify C1QTNF1-AS1 protein interactors
We used C1QTNF1-AS1 antisense oligonucleotide pulldown coupled with MS to identify protein interactors of lncRNA C1QTNF1-AS1 in human HCT116 cells. Five antisense oligonucleotides against C1QTNF1-AS1 and luciferase (negative control) were used for the pulldown. Three independent oligonucleotides (C...
ORGANISM(S): Homo sapiens (Human) 
2026-03-12 | PXD059546 | Pride
Antisense oligo pulldown  rna interactome  lc-ms/ms  label-free quantification 
MetabolomicsOsmolality-based normalization enhances statistical discrimination of untargeted metabolomic urine analysis: results from a comparative study

INTRODUCTION: Because of its ease of collection, urine is one of the most commonly used matrices for metabolomics studies. However, unlike other biofluids, urine exhibits tremendous variability that can introduce confounding inconsistency during result interpretation. Despite man...

2023-09-04 | MTBLS1765 | MetaboLights
ProteomicsProteomic analysis of plasma membrane and tonoplast from the leaves of a mangrove plant Avicennia officinalis
We used gel electrophoresis coupled with LC-MS based proteomics to identify key transport proteins in the plasma membrane (PM) and tonoplast fractions of Avicennia officinalis leaves. This was part of our attempts to understand salt tolerance and secretion in mangrove plant species. PM and tonoplast...
ORGANISM(S): Avicennia 
2014-11-18 | PXD000837 | Pride
Tonoplast Plasma membrane Proteomics Avicennia officinalis Salt secretion 
UnknownUsing shape to turn off blinking for two-colour multiexciton emission in CdSe/CdS tetrapods.
Not available
S-EPMC5437295 | biostudies-literature
Sort   by:  
 Page size