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ProteomicsDrosophila variant Polycomb complex subunits LC-MS
Our proteomics anaylsis in Drosophila embryos revealed alternative forms of PRC2 and several distinct variant PRC1 types which are similar to polycomb complexes in mammals
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-10-15 | PXD033615 | Pride
Drosophila vprc1 Lc-ms Biotap-xl Prc2.2 Polycomb 
ProteomicsOncogenic BRD4-NUT protein interactions
To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma, we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared to wild type BRD4. Using crosslinking, affinity purification, and mass spectrometry, we identified ...
ORGANISM(S): Homo sapiens (Human) 
2017-04-05 | PXD005786 | Pride
Biotap-xl Znf532-nut Hyperacetylation Topological domains 
TranscriptomicsHeterochromatin-associated interactions of Drosophila HP1 with dADD1, HIPP1, and repetitive RNAs
We report chromatin-associated protein and RNA interactions of HP1a, indentified by BioTAP-XL mass spectrometry and sequencing. We identify an extensive list of both known and novel HP1a-interacting proteins from Drosophila S2 cells and from whole organisms across embryonic, larval and adult stages....
ORGANISM(S): Drosophila melanogaster 
2014-07-03 | E-GEOD-56101 | biostudies-arrayexpress
GenomicsReciprocal interactions of human C10orf12 and C17orf96 with PRC2 revealed by BioTAP-XL crosslinking and affinity purification
Short-read sequencing of DNA fractions of the BioTAP-XL pull-downs on human EZH2 and interacting proteins. ChIP-seq-like pulldown/input samples
ORGANISM(S): Homo sapiens 
2014-02-01 | E-GEOD-53495 | biostudies-arrayexpress
TranscriptomicsMSL complex is attracted to genes marked by H3K36 trimethylation using a sequence-independent mechanism
In Drosophila, X chromosome dosage compensation requires the male-specific lethal (MSL) complex, which associates with actively transcribed genes on the single male X chromosome to upregulate transcription approximately 2-fold. We found that on the male X chromosome, or when MSL complex is ectopical...
ORGANISM(S): Drosophila melanogaster 
2007-09-20 | E-GEOD-8557 | biostudies-arrayexpress
TranscriptomicsDrosophila MSL complex globally acetylates H4 Lys16 on the male X chromosome for dosage compensation
Drosophila MSL complex binds the single male X chromosome to upregulate gene expression to equal that from the two female X chromosomes. However, it has been puzzling that ~25% of transcribed genes on the X do not stably recruit MSL complex. Here, we find that almost all active genes on the X are ...
ORGANISM(S): Drosophila melanogaster 
2009-06-10 | E-GEOD-14884 | biostudies-arrayexpress
TranscriptomicsThe MSL3 chromodomain directs a key targeting step for dosage compensation of the Drosophila
Active genes on the X chromosome of Drosophila males are upregulated by the Male-specific lethal (MSL) complex containing five MSL proteins and two non-coding roX RNAs. To probe the targeting mechanism, we have solved the structure of the MSL3 chromodomain, designed point mutations in key residues t...
ORGANISM(S): Drosophila melanogaster 
2008-11-18 | E-GEOD-11817 | biostudies-arrayexpress
TranscriptomicsX chromosome dosage compensation via enhanced transcriptional elongation in Drosophila males (Control & MSL2 RNAi)
MSL (Male-specific lethal) complex increases transcription on the single X chromosome of Drosophila males in order to equalize expression of X-linked genes between males (XY) and females (XX). The increase in transcript levels correlates with MSL- dependent acetylation of histone H4 at K16 within th...
ORGANISM(S): Drosophila melanogaster 
2011-03-03 | E-GEOD-25887 | biostudies-arrayexpress
TranscriptomicsX chromosome dosage compensation via enhanced transcriptional elongation in Drosophila males (Untreated)
MSL (Male-specific lethal) complex increases transcription on the single X chromosome of Drosophila males in order to equalize expression of X-linked genes between males (XY) and females (XX). The increase in transcript levels correlates with MSL- dependent acetylation of histone H4 at K16 within th...
ORGANISM(S): Drosophila melanogaster 
2010-12-22 | E-GEOD-25321 | biostudies-arrayexpress
GenomicsdMi-2(Q4443).14-16 hr OR embryo.Solexa
modENCODE_submission_5070 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under s...
ORGANISM(S): Drosophila melanogaster 
2013-05-28 | E-GEOD-47265 | biostudies-arrayexpress
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