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MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Simulated)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS235 | MetaboLights
MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Plasma)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS234 | MetaboLights
UnknownLabel-Free Quantification from Direct Infusion Shotgun Proteome Analysis (DISPA-LFQ) with CsoDIAq Software.
Not available
S-EPMC9850400 | biostudies-literature
ProteomicsLFQbench enables a multi-centered benchmark study demonstrating robust proteomic label-free quantification
The consistent and accurate quantification of proteins is a challenging task for mass spectrometry (MS)-based proteomics. SWATH-MS uses data-independent acquisition (DIA) for label-free quantification. Here we evaluated five software tools for processing SWATH-MS data: OpenSWATH, SWATH2.0, Skyline, ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2016-09-27 | PXD002952 | Pride
Lfqbench Lfq Swath-ms Benchmark 
ProteomicsLabel-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using CE-ESI-HRMS
In this work, we integrate capillary electrophoresis electrospray ionization mass spectrometry, bottom-up proteomics, and single-cell microanalysis to enable the label-free quantification (LFQ) of proteins in single embryonic cells (D11 blastomeres) in the 16-cell frog (Xenopus laevis) embryo. Based...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2016-06-23 | PXD004174 | Pride
Blastomere Embryo Proteomics Xenopus Mass spectrometry Fusion Label free quantification Single cell Lfq Capillary electrophoresis Electrospray ionization Impact hd 
ProteomicsLabel-free Quantification of Host-Cell Protein Impurity in a Recombinant Hemoglobin Reference Material
Quantitative analysis depends on pure-substance primary calibrators with known mass fractions of impurity. The datasets included were generated using mass spectrometry for the evaluation of label-free quantification (LFQ) as a method for determining the mass fraction of host-cell proteins (HCPs) in...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2023-05-09 | PXD041736 | Pride
Protein reference materials Dda-pasef Label-free quantification Host-cell proteins 
ProteomicsIdentification of stably expressed reference genes for expression studies in Arabidopsis thaliana using mass spectrometry-based label-free quantification
Arabidopsis thaliana has been used regularly as a model plant in gene expression studies on transcriptional reprogramming upon pathogen infection, such as that by Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), or when subjected to stress hormone treatments including jasmonic acid (JA), salicyl...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-09-05 | PXD035677 | Pride
Pathogen infection  jasmonic acid (ja)  salicylic acid (sa)  abscisic acid (aba)  expression study  label-free quantification (lfq)  rt-qpcr  reference gene 
ProteomicsRobust collection and processing for label-free single voxel proteomics
With advanced mass spectrometry (MS)-based proteomics, genome-scale proteome coverage can be achieved from bulk tissues. However, such bulk measurement lacks spatial resolution and obscures important tissue heterogeneity, which make it impossible for proteome mapping of tissue microenvironment. Here...
ORGANISM(S): Homo Sapiens (human) 
2024-11-01 | PXD045043 | JPOST Repository
ProteomicsLabel-Free Quantification Method for Assessing Sex from Modern and Ancient Bovine Tooth Enamel
Identification of the sex of fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of fossils is often impossible. To overcome this, we have developed an innovative proto...
ORGANISM(S): Bos taurus (Bovine) 
2024-10-17 | PXD053146 | Pride
Bovine Amels Sex determination 
UnknownLabel-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears.
Not available
S-EPMC9225558 | biostudies-literature
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