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ProteomicsCombining label-free and label-based accurate quantifications with SWATH-MS: comparison with SRM and PRM for the evaluation of bovine muscle type effects.
Mass spectrometry has proven to be a valuable tool for the accurate quantification of proteins. In this study, we have evaluated the performances of three targeted approaches, namely Selected Reaction Monitoring (SRM), Parallel Reaction Monitoring (PRM) and Sequential Windowed Acquisition of Theoret...
ORGANISM(S): Bos Taurus 
2021-06-08 | PXD020680 | panorama
ProteomicsCombining label-free and label-based accurate quantifications with SWATH-MS: comparison with SRM and PRM for the evaluation of bovine muscle type effects
The performances of label-based SWATH-MS, SRM and PRM to accurately quantify ten candidate biomarkers of beef meat tenderness or marbling, in a cohort of 64 bovine muscle tissues expected to cover a wide biological range of these traits, were evaluated. Limits of quantification, dynamic range and qu...
ORGANISM(S): Bos taurus (Bovine) 
2021-06-09 | PXD020638 | Pride
Beef meat quality  data-independent acquisition (dia)  quantitative ms  swath-ms  targeted proteomics 
UnknownSerum proteomics analysis of feline mammary carcinoma based on label-free and PRM techniques.
Not available
S-EPMC7263907 | biostudies-literature
ProteomicsPRM for JEV infected BV2 cells proteomics
Quantification of Differentially Expressed Proteins (DEPs) by PRM For the JEV-infected group, 15 upregulated DEPs were selected for validation by PRM. Protein extraction and tryptic digestion were performed following the same protocol used for the label-free quantitative proteomics experiment.
ORGANISM(S): Mus musculus (Mouse) 
2022-09-09 | PXD035938 | Pride
Prm Jev Bv2 
ProteomicsDiscovery and validation of novel protein biomarkers in ovarian cancer patient urine
Diagnosis of ovarian cancer at an early stage is the most important determinant of survival. Thus, there is a clear need for novel biomarkers to improve diagnostic and prognostics that may better inform on therapeutic strategies. We have conducted a discovery study using label-free quantitative mass...
ORGANISM(S): Homo sapiens (Human) 
2018-02-15 | PXD007962 | Pride
Urine Prm Ovarian cancer Lfq He4 Parallel reaction monitoring Label free quantitation 
ProteomicsDrosophila melanogaster proteome dynamics during embryogenesis - 1
In this study we use a combination of proteomics Label-Free quantification methods to monitor protein expression changes over a time course of more than 20 hours of embryo development in Drosophila melanogaster.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-04-11 | PXD003178 | Pride
Drosophila melanogaster  label-free quantification  swath 
UnknownLabel-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats.
Not available
S-EPMC11236725 | biostudies-literature
ProteomicsAn Integrated Quantitative Proteomics Workflow for Biomarker Discovery and Validation in Plasma
Blood plasma is one of the most widely used samples for biomarker discovery research as well as clinical investigations for diagnostic and therapeutic purposes. However, the plasma proteome is extremely complex due to its wide dynamic range of protein concentrations and the presence of high-abundanc...
ORGANISM(S): Homo sapiens (Human) 
2020-09-07 | PXD017834 | Pride
Targeted proteomics Label-based quantitation Label-free quantitation 
ProteomicsTargeted proteomics of signal pathways in BV2 microglial cell activation
Microglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, using label-free PRM approach which is allowing ...
ORGANISM(S): Mus musculus (Mouse) 
2017-08-09 | PXD006556 | Pride
Targeted protoemics Quantitative analysis Label-free prm Bv2 activation 
ProteomicsLabel-Free proteomic analysis and functional analysis in patients with intrauterine adhesion
Intrauterine adhesion is a common disease in women of childbearing age. In recent years, due to the increasing number of uterine operations, the incidence has been increasing, resulting in rising rate of secondary infertility in women. At present, the mechanism of intrauterine adhesion is still uncl...
ORGANISM(S): Homo Sapiens 
PXD036768 |
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