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Mouse sample preparation: 'Severe' SMA mice (Smn-/-;SMN2+/+) and wild-type (Smn+/+;SMN2+/+) littermates at postnatal day 5 (P5) were sacrificed by chilling on ice and decapitation. Levator auris longus (LAL, from the back of the neck) muscles were dissected in oxygenated mammalian physiological s...
ORGANISM(S): Mus musculus (Mouse) 
2013-10-09 | PXD000488 | Pride
The project aims to unveil the mechanism of rifamycin and its analog production by wild type A. mediterranei S699 and mutant A. mediterranei DCO#34 known to produce 24-desmethylrifamycin B at two time points. Stain that donot produce this molecule is taken as the negative control in the study.
ORGANISM(S): Amycolatopsis mediterranei S699 
2021-03-11 | PXD016416 | Pride
Gene expression analysis of S. coelicolor M145 wild type strain, and mutants delta0877 and delta 7173. RNA samples were extracted from 48 h culture samples during growth in flasks in defined mineral medium MG supplemented with 5% of yeast extract. Cy5 labelled genomic DNA was used as the common ref...
ORGANISM(S): Streptomyces coelicolor 
The goal of this study was to determine developmental differences in gene expression between left and right ventricle, and to assess the differential effect of altered hemodynamic loading on left and right ventricle. Chick ventricles from different developmental stages were isolated for assessment o...
ORGANISM(S): Gallus gallus 
Lysosomal acid lipase (LAL) is the key enzyme of lysosomal lipid hydrolysis, which degrades cholesteryl esters (CE), triacylglycerols (TG), diacylglycerols (DG), and retinyl esters. The role of LAL in various cellular processes has mostly been studied in LAL-deficient (Lal-/-) mice, which share phen...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-13 | PXD030672 | Pride
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