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UnknownLC-ESI/LTQ-Orbitrap-MS Based Metabolomics in Evaluation of Bitter Taste of Arbutus unedo Honey.
Not available
S-EPMC8125850 | biostudies-literature
UnknownLC-ESI(±)-LTQ MS<sup>n</sup>-Based Metabolomic Profiling of Coffee: Fragmentation Pathways for Identification of Major Polar Compounds.
Not available
S-EPMC12142669 | biostudies-literature
UnknownGlobal relative quantification with liquid chromatography-matrix-assisted laser desorption ionization time-of-flight (LC-MALDI-TOF)--cross-validation with LTQ-Orbitrap proves reliability and reveals complementary ionization preferences.
Not available
S-EPMC3790300 | biostudies-literature
ProteomicsProteomic analysis of subchloroplastic compartments
Arabidopsis thaliana chlorosplasts extraction followed by subchloroplastic compartments fractionation (envelope, stroma, thylakoids). Afer SDS-PAGE separation and trypsin digestion, analysis of all gel bands twice by LC-MS/MS on a LTQ-FT
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-23 | PXD000136 | Pride
Sub compartments fractionation Chloroplast Ltq-ft Lc-ms/ms 
ProteomicsA shotgun proteomics reference map for the alkali-sensitive covalently-linked proteins of the cell wall of A. fumigatus during specific stages of early fungal development (dormant conidia, isotropically expanding conidia, early germ tube emergence and young hyphae).
Four different developmental time points representing the Afu development from dormant conidia into young hyphae (0h, 4h, 6h, 8h) were chosen. Cell walls of the Afu samples were extracted using denaturing methods followed by isolation of solubilized proteins. Samples were processed using filter aide...
ORGANISM(S): Aspergillus fumigatus Af293 
2016-12-20 | PRD000567 | Pride
UnknownSystematic screening and structural characterization of dipeptides using offline 2D LC-LTQ-Orbitrap MS: A case study of Cordyceps sinensis.
Not available
S-EPMC9091903 | biostudies-literature
ProteomicsArabidopsis cell LC-MSMS - Proteomics analysis reveals a highly heterogeneous proteasome composition and the post-translational regulation of peptidase activity under pathogen signaling in plants
26S proteasome was affinity-purified with anti-FLAG beads and separated by 2-DE method. Each spot was cut and digested by Trypsin enzyme, which finaly applied for LC-MS/MS nalysis using a LTQ-Orbitrap XL (Thermo Scientific). The obtained spectra were compared against 35,386 sequences in The Arabidop...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-07-28 | PXD000417 | Pride
26sp 
UnknownPerformance comparisons of nano-LC systems, electrospray sources and LC-MS-MS platforms.
Not available
S-EPMC3886563 | biostudies-literature
ProteomicsIdentification of Autocrine Growth Factors Secreted by CHO cells for Applications in Single Cell Cloning Media
To identify low abundance autocrine growth factors in CHO cell conditioned media, we utilized a label-free shotgun proteomics approach. CHO cell conditioned media were harvested from fed batch bioreactors and concentrated using methanol/chlorofrom precipitation. Proteins in the samples were then su...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-01-22 | PXD000214 | Pride
Secreted proteins Chinese hamster ovary cells Shotgun proteomics Autocrine growth factors 
UnknownUltrasensitive characterization of site-specific glycosylation of affinity-purified haptoglobin from lung cancer patient plasma using 10 μm i.d. porous layer open tubular liquid chromatography-linear ion trap collision-induced dissociation/electron transfer dissociation mass spectrometry.
Not available
S-EPMC3073582 | biostudies-literature
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