To investigate how the sucrose-TOR signaling pathway regulates phyA, we conducted LC-MS/MS analysis to identify phosphorylation sites on phyA. In vivo, the phyA protein was immunoprecipitated from 35S::PHYA-YFP/phyA-211 seedlings treated with shade and 30 mM sucrose for 6 hours. In vitro, we used r...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress)