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ProteomicsHuman liver cell line LO2 LC-MS/MS
A hyperlipidaemia model of L02 cells was established using free fatty acids (FFA, oleate and palmitate, 2:1; Sigma-Aldrich, USA) at a final concentration of 1 mM for 24 h. Cells were allowed to attach overnight prior to HTG-medicated serum for 24 h and then exposed to FFA during drug treatment. Afte...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD045069 | Pride
Human Liver cell Lc-ms/ms 
ProteomicsIdentification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.
GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The...
ORGANISM(S): Homo sapiens (Human) 
2024-06-17 | PXD050141 | Pride
Keratin  krt32 
ProteomicsDe novo designed protein guiding targeted protein degradation
Targeted protein degradation is a powerful tool for biological research, cell therapy, and synthetic biology. However, conventional methods often depend on pre-fused degrons or chemical degraders, limiting their wider applications. In this study, a guided protein labeling and degradation system (GPl...
ORGANISM(S): Escherichia coli 
2025-07-05 | PXD063956 | Pride
Protein degradation  protein design 
ProteomicsDysregulation of N-glycosylation by RPN1 knockout in spermatocytes induces male infertility via impaired mitochondrial function in mice
N-glycosylation protein modifications play a crucial regulatory role in numerous biological processes, although their contribution to male reproduction in mammals remains largely undefined. Here, we found that Ribophorin I (RPN1), a subunit of oligosaccharyltransferase complex, is indispensable for ...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD051379 | Pride
Rpn1  n-glycosylation  meiosis  spermatogenesis  fertility 
ProteomicsSerum proteomics of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet
The serum samples from wild type mice fed high-fat diet for 12 weeks (WT_Serum) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (KI_Serum) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics tec...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-11 | PXD034069 | Pride
Serum Proteomics Mice Wild type Mdm2 
ProteomicsThe eWAT proteomics of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet
The epididymal adipose tissue (eWAT) samples from wild type mice fed high-fat diet for 12 weeks (H_WT_E) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (H_KI_E) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectromet...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-11 | PXD034074 | Pride
Proteomics Ewat Mice Mdm2 High-fat diet 
UnknownMapping the N-linked glycosites of rice (Oryza sativa L.) germinating embryos.
Not available
S-EPMC5362090 | biostudies-literature
ProteomicsUBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice
For proteomics analysis, testes of Ube2j2-/- and WT mice were collected on ice, washed once with pre-cooled PBS, dried with absorbent paper, and immediately placed in liquid nitrogen, dry ice, or at -80 ℃. Samples were packed in dry ice and transported to Hangzhou Jingjie Biotechnology Company (PTM ...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-05 | PXD063637 | Pride
Meiosis arrest Synaptonemal complex disassembly. Ube2j2 Homologous recombination 
UnknownHistone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.
Not available
S-EPMC7857430 | biostudies-literature
UnknownSystems Pharmacology and In Silico Docking Analysis Uncover Association of CA2, PPARG, RXRA, and VDR with the Mechanisms Underlying the Shi Zhen Tea Formula Effect on Eczema.
Not available
S-EPMC8143894 | biostudies-literature
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