For metabolic profiling of human CD4+ T cells，stable isotope labeling (SIL) and metabolomics SIL experiments were performed with in vitro-activated T cells using liquid chromatography (LC) coupled to mass spectrometry (MS).
For metabolic profiling of mouse CD4+ T cells, stable isotope labeling (SIL) experiments were performed with in vitro-activated T cells using gas chromatography (GC) coupled to mass spectrometry (MS).
Excessive salt intake is a widespread health issue observed in almost every country around the world. A high salt diet (HSD) has a strong correlation with numerous diseases, including hypertension, chronic kidney disease and autoimmune disorders. However, the mechanisms underlying HSD-promotion o...
We performed a combination of metabolic engineering (deletion of ldh and poxB and overexpression of mmc) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in Propionibacterium acidipropionici. The results indicated that the mutants had superior physiologic...
Alkaline salts (e.g., NaHCO3 and Na2CO3) causes more severe morphological and physiological damage to plants than neutral salts (e.g., NaCl and Na2SO4) due to differences in pH. The mechanism by which plants respond to alkali stress is not fully understood, especially in plants having symbotic relat...
In this study, we performed the first large-scale nuclear proteome analysis of wheat developing grains under water deficit by label-free based quantitative proteomic approach.
K562 cell line treated with 1,6-HD and proteins were captured via cross-linking. We quantified chromatin structure changes and chromatin binding proteins in K562 cell before and after 1,6-hexanediol treatment by Hi-C and Hi-MS, respectively.
