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The regulation of the photosynthetic apparatus in higher plants is highly connected to the organization of the thylakoid membrane into appressed and non-appressed regions. The two photosystems (I and II) are the spatially strongest segregated protein complexes by this thylakoid heterogenous organiza...
ORGANISM(S): Arabidopsis Thaliana 
2019-11-01 | PXD014562 | panorama
Arabidopsis thaliana Col-0 plants were grown under different light conditions. Chloroplasts were isolated and their proteins extracted. Proteins were analysed by LC-MS and a label free quantification strategy was used to compare the different proteome compositions responsible for the respective accl...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
The plant chloroplast thylakoid membrane must respond to environmental variations in light intensity to maximise the efficiency of photosynthesis and minimise photo-oxidative stress. Plants respond to changing light intensity in the short-term (seconds to minutes) through altered regulation of the s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-08-23 | PXD014982 | Pride
Plant thylakoid membranes contain hundreds of proteins closely interplaying to cope with ever-changing environmental conditions. We investigated how P. sativum (pea) grown at different irradiances optimizes light-use efficiency through the differential accumulation of thylakoid proteins. Thylakoid m...
ORGANISM(S): Pisum sativum (Garden pea) 
2018-10-02 | PXD009448 | Pride
Typically, when fully developed leaves of Arabidopsis thaliana are exposed to an increase in light intensity, they are able to increase their photosynthetic capacity in a process known as dynamic acclimation. Fully developed leaves of Arabidopsis thaliana were exposed to a fourfold increase in light...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2017-11-14 | MSV000081715 | MassIVE
Biogenesis and maintenance of the photosynthetic thylakoid membrane requires transport of lipids from their site of synthesis in the chloroplast envelopes to their destination in the thylakoid. While vesicle trafficking is likely involved, we hypothesized a complementary mechanism involving direct m...
ORGANISM(S): Pisum sativum (Garden pea) 
2026-04-29 | PXD065686 | Pride
Thylakoid membranes are the specialized internal membrane system produced in plants, algae, and cyanobacteria to convert sunlight to chemical energy via oxygenic photosynthesis. Cyanobacterial thylakoid membranes harbor the protein complexes and electron transport molecules that are necessary for ph...
ORGANISM(S): Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) 
2020-11-17 | PXD019731 | Pride
Photosynthesis relies on a dynamic network of protein-protein interactions that adapt to fluctuating light conditions. We present an XL-MS pipeline applied directly to functional thylakoid membranes from Arabidopsis thaliana and Spinacia oleracea. By combining PhoX crosslinker with the amphiphilic s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Spinacia sp. 
2026-05-14 | PXD068425 | Pride
Plants grown under different illumination levels undergo a long-term acclimation response (LTR) resulting in differences in their leaf morphology and chloroplast protein composition to maximise their growth. At the proteomic level, LTR involves modulating the stoichiometry of photosynthetic complexe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-03-30 | PXD037358 | Pride
The thylakoid membrane in land plants is organized into an appressed domain (grana), enriched in photosystem (PS) II, and a non-appressed domain (stroma lamellae) enriched in PSI. This ultrastructure ensures control over excitons spillover from PSII to PSI. The bulky machinery required for the repai...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-02-11 | PXD046544 | Pride
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