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ProteomicsLimited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells
To study protein structures directly within cells, we developed in-cell limited proteolysis-coupled mass spectrometry (in-cell LiP-MS). Conditions for introduction of proteinase K into human cells using electroporation were optimized and validated for intracellular cleavages. In-cell LiP-MS captured...
ORGANISM(S): Homo sapiens (Human) 
2025-11-09 | PXD069095 | Pride
Stress granules Mass spectrometry In-cell lip Structural proteomics Limited proteolysis Arsenite treatment Rapamycin 
ProteomicsCortisol binding sites on SARS-CoV-2 S1 identified by limited proteolysis-coupled mass spectrometry
We used a limited proteolysis-coupled mass spectrometry approach to pinpoint binding sites of cortisol on SARS-CoV-2 S1. Binding target identification is based on the principle that small-ligand binding alters (increases or decreases) the protease accessibility of the target protein37,38. The bindin...
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 
2022-06-10 | PXD032937 | Pride
Limited proteolysis Lc-ms Cortisol Covid-19 Sars-cov2 spike s1 
UnknownAnalysis of Limited Proteolysis-Coupled Mass Spectrometry Data.
Not available
S-EPMC12036054 | biostudies-literature
ProteomicsDiscovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry
Jasmonic acid (JA) is the precursor of the plant hormone, jasmonoyl-isoleucine, with roles in defences and development. Molecular signalling pathways of JA are well-understood, but it is still not fully understood how JA is metabolised. We used limited proteolysis-coupled mass spectrometry to search...
ORGANISM(S): Solanum lycopersicum 
2026-08-27 | PXD057493 | Pride
Jasmonic acid Lip-ms Protein-metabolite interactome Tomato 
UnknownBenchmarking of Quantitative Proteomics Workflows for Limited Proteolysis Mass Spectrometry.
Not available
S-EPMC12022698 | biostudies-literature
ProteomicsBenchmarking of quantitative proteomics workflows for Limited proteolysis mass spectrometry
Limited proteolysis coupled with mass spectrometry (LiP-MS) has emerged as a powerful technique for detecting protein structural changes and drug-protein interactions on a proteome-wide scale. However, there is no consensus on the best quantitative proteomics workflow for analyzing LiP-MS data. In t...
ORGANISM(S): Homo sapiens (Human) 
2025-03-31 | PXD055927 | Pride
Dia Tmt Limited proteolysis Staurosporine 
UnknownLimited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome.
Not available
S-EPMC7824793 | biostudies-literature
UnknownDiLeu Isobaric Labeling Coupled with Limited Proteolysis Mass Spectrometry for High-Throughput Profiling of Protein Structural Changes in Alzheimer's Disease.
Not available
S-EPMC10330787 | biostudies-literature
UnknownA comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry.
Not available
S-EPMC4534179 | biostudies-literature
ProteomicsAnalysis of limited proteolysis-coupled mass spectrometry data
Limited proteolysis combined with mass spectrometry (LiP-MS) facilitates probing structural changes on a proteome-wide scale. This method leverages differences in the proteinase K accessibility of native protein structures to concurrently assess structural alterations for thousands of proteins in si...
ORGANISM(S): Schizosaccharomyces pombe 927 
2025-05-05 | PXD055352 | Pride
Yeast Dia Lip-ms Lc-ms/ms S.pombe 
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