Sort   by:  
 Page size 
MetabolomicsAn NMR metabolomics analysis pipeline for human neutrophil samples with limited source material
Untargeted 1H NMR metabolomics is a robust and reproducible approach to study metabolism in biological samples, providing unprecedented insight into altered cellular processes associated with human diseases. Metabolomics is increasingly used alongside other -omics techniques, such as proteomics and ...
2025-11-06 | MTBLS6220 | MetaboLights
ProteomicsThe $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers
Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To democratize access to proteomics and facilitate its integration into more high-throughput multiomic...
ORGANISM(S): Homo sapiens (Human) 
2026-02-04 | PXD066701 | Pride
Limited sample input $10 proteome 
ProteomicsFully automated sample processing and analysis workflow for low-input proteome profiling
Recent advances in sample preparation and analysis have enabled direct profiling of protein expression in single mammalian cells and other trace samples for characterization of cellular heterogeneity and high-resolution mapping of tissues. Several techniques used to prepare and analyze low-input sam...
ORGANISM(S): Homo sapiens (Human) 
2020-12-21 | PXD021882 | Pride
Low-input sample preparation Low-input proteomics 
ProteomicsoptiPRM: A targeted immunopeptidomics LC-MS workflow with ultra-high sensitivity for the detection of mutation-derived tumor neoepitopes from limited input material
Personalized cancer immunotherapies such as vaccines and T cell receptor (TCR)-transgenic T cells rely on the presentation of tumor-specific peptides by human leukocyte antigen (HLA) class I molecules to cytotoxic T cells. Such neoepitopes can for example arise from somatic mutations and their ident...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD043542 | Pride
Immunopeptidomics Prm Lc-ms Targeted ms 
ProteomicsSample Size-comparable Spectral Library Enhances Data-Independent Acquisition-based Proteome Coverage of Low-input Cells
Despite advancement of data-independent acquisition mass spectrometry (DIA-MS) to provide comprehensive and reproducible quantitative proteome profiling, its utility in very low-input samples is limited. For low-input samples at microscale, the proteome composition and overall peptide ion patterns a...
ORGANISM(S): Homo Sapiens (human) 
2022-11-07 | PXD027679 | JPOST Repository
ProteomicsA Suspension Trapping-based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2023-08-28 | PXD039933 | JPOST Repository
TranscriptomicsLow input Hi-C datasets for a diffuse large B-cell lymphoma sample, healthy control human B-cells and mouse embryonic stem cells
In this experiment, we've examined chromatin conformation of OG2 (B6; CBA-Tg(Pou5f1-EGFP)2Mnn/J; stock number 004654) mouse stem cells cultured as described in (Shi et al., 2008), using different amounts of starting cells. We performed a modified in situ Hi-C protocol for 6 samples digested with Mbo...
ORGANISM(S): Mus musculus 
2018-11-30 | E-MTAB-5875 | biostudies-arrayexpress
TranscriptomicsLimited systemic sclerosis patients with PAH show biomarkers of inflammation and vascular injury
Limited systemic sclerosis patients with pulmonary arterial hypertension show biomarkers of inflammation and vascular injury Forty-nine PBMC samples were obtained from 21 lSSc subjects without PAH (lSSc-noPAH), 15 lSSc subjects with PAH (lSSc-PAH), and 10 healthy controls; three subjects provided P...
ORGANISM(S): Homo sapiens 
2010-10-27 | E-GEOD-19617 | biostudies-arrayexpress
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-10 | PXD011323 | Pride
Interaction proteomics Low input Ap-ms 
UnknownRibo-ITP expands the translatome of limited input samples.
Not available
S-EPMC12340811 | biostudies-literature
Sort   by:  
 Page size