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ProteomicsPhosphoproteome analysis
The phosphatase activirty and dephosphorylation site are determined by using the recombinant protein. After treatment, the level of specific phosphorylation site is decreased. Also, the biological importance is evaluated through a serial of experiments including immunoprecipitation, western blotting...
ORGANISM(S): Homo sapiens (Human) 
2022-04-13 | PXD002894 | Pride
Tyrosine phosphorylation Lc-ms/ms 
ProteomicsBacterial phosphoproteome - Systematic profiling of the bacterial phosphoproteome reveals bacterium-specific features of phosphorylation
Protein phosphorylation is a crucial post-translational modification in bacteria, but has not been extensively studied because of the technical difficulty of phosphopeptide enrichment. We present a new enrichment protocol, approximately 10 times more efficient than conventional approaches in E. coli...
ORGANISM(S): Escherichia coli 
2015-09-22 | PXD001264 | Pride
Protein phosphorylation Lc-ms/ms 
ProteomicsK. pneumoniae phosphoproteome - Systematic profiling of the bacterial phosphoproteome reveals bacterium-specific features of phosphorylation
Highly efficient phosphopeptide enrichment developed for bacterial phosphoproteomics. The most comprehensive phosphoproteme maps in both gram-positive/negative bacteria. Discovery of phosphorylation motifs across distinct bacterial species
ORGANISM(S): Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044 
2015-09-22 | PXD001263 | Pride
Phosphorylation K. pneumoniae Lc-msms 
ProteomicsB. subtilis phosphoproteome - Systematic profiling of the bacterial phosphoproteome reveals bacterium-specific features of phosphorylation
Highly efficient phosphopeptide enrichment developed for bacterial phosphoproteomics. The most comprehensive phosphoproteme maps in both gram-positive/negative bacteria. Discovery of phosphorylation motifs across distinct bacterial species
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2015-09-22 | PXD001298 | Pride
B. subtilis Lc-msms Phosphorylation 
ProteomicsFlagellin (FliC) phosphorylation affects Type 2 protease secretion and biofilm dispersal in Pseudomonas aeruginosa PAO1
Protein phosphorylation has a major role in controlling the life-cycle and infection stages of bacteria. Proteome-wide occurrence of S/T/Y phosphorylation has been reported for many prokaryotic systems. Previously, we reported the phosphoproteome of Pseudomonas aeruginosa and Pseudomonas putida. In ...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2022-03-02 | PXD003645 | Pride
Phosphorylation Flagellin Lc-msms 
ProteomicsActive DNA Demethylation by DNMT3A and DNMT3B - Mechanistic Aspect and In Vivo Evidence
The DNA methylation program is at the bottom layer of the epigenetic regulatory cascade of vertebrate development. While the methylation at C-5 position of the cytosine (C) residues on the vertebrate genomes is achieved through the catalytic activities of the DNA methyltransferases (DNMTs), the...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-24 | PXD003401 | Pride
Methylation Lc-msms 
ProteomicsBenchmarking differential expression, imputation and quantification methods for proteomics data
Data analysis is a critical part of quantitative proteomics studies in interpreting biological questions. Numerous computational tools including protein quantification, imputation, and differential expression (DE) analysis were generated in the past decade. However, searching optimized tools is stil...
ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast) Drosophila Melanogaster (fruit Fly) 
2022-11-16 | PXD029891 | JPOST Repository
ProteomicsUniversal and robust bacterial phosphoproteomics analysis
In present work, we established a simple and robust protocol for universal bacterial phosphoproteomic analysis underlying the rationale of methanol-based LLE protein extraction and cleanup. Using the SDS lysis as the conventional protocol control, we carefully evaluated the efficiency of protein ext...
ORGANISM(S): Escherichia Coli Cellular Organisms 
2025-08-04 | PXD055159 | JPOST Repository
ProteomicsComparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied with Fibrosis in Human Stroma
Cornea transplantation is a widely performed procedure globally. However, storing donor corneas for extended periods in Optisol-GS storage medium at 4 ℃ can lead to reduced endothelial cell viability, causing severe stromal edema and opacification. This negatively impacts transplantation success. Th...
ORGANISM(S): Homo Sapiens (human) 
2024-09-29 | PXD045907 | JPOST Repository
ProteomicsCold Atmospheric Plasma Jet Activated Medium Promotes Wound Healing through CK2-mediated ERK and AKT signaling pathways
In this study, we aimed to explore the molecular mechanisms of wound healing affected by CAPJ using the parallel MS-based proteome and phosphoproteome. The keratinocyte, HaCaT, was used as cell mode to treat CAPJ indirectly by employing plasma activated medium (PAM) which was culture medium explored...
ORGANISM(S): Homo Sapiens (human) 
2025-02-22 | PXD050065 | JPOST Repository
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