Background: Semen Cuscutae flavonoids (SCFs) constitute a class of metabolites of Semen Cuscutae, a botanical drug that was recently found to have an anti-depression effect. This study aimed to evaluate the anti-depression effects of SCFs in chronic unpredi...
This project investigates the proteomic changes in Aeromonas hydrophila under potassium dichromate-induced stress using both data-dependent acquisition (DDA) and data-independent acquisition (DIA) LC-MS/MS strategies. Protein extracts from wild-type and dichromate-treated A. hydrophila cells were en...
Transcriptome analysis of mouse embryonic stem cell lines derived from embryos cultured in optimal and suboptimal conditions compared to cell lines derived from control embryos. The use of assisted reproductive technologies (ART) such as in vitro fertilization (IVF) has resulted in the birth of more...
Filip Mundt, Sandeep Rajput, Shunqiang Li, Kelly Ruggles, Arshag D. Mooradian, Philipp Mertins, Michael A. Gillette, Karsten Krug, et al., Cancer Res. 2018 May 15;78(10):2732-2746. doi: 10.1158/0008-5472.CAN-17-1990.
Activation of phosphoinositide 3-kinase (PI3K) signaling is frequently obs...
In this work, we have developed a simplified, fast and efficient EVs-based proteomics strategy to enable biomarker discovery for early diagnosis of CRC. By covalent conjugation of an EV-targeting probe, DSPE, to agarose beads, we achieved one-step enrichment of EVs from plasma fluids in 10 minutes w...
Jujube is an important medicinal plant and economic crop in China. However, long-term infestation by jujube witches’ broom disease has led to significant yield losses. In this study, TMT-based quantitative proteomics was employed to investigate the molecular mechanisms underlying disease resistance...
To identify human DTX2 proximal proteome, the BioID-DTX2 fusion protein was expressed in DTX2 KO HeLa cells. The biotinylated proteins were pulled down and subjected to mass spectrometry analysis.
