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ProteomicsCross-linking mass spectrometry of yeast mitochondria grown on different carbon sources reveal Ndi1, Min8 and Pet9 associated with the electron transport chain
Mitochondria derived from Saccharomyces cerevisiae grown on either a non-fermentable (glycerol) or a fermentable (glucose) carbon source were cross-linked with BS3. Additionally, by using a stable-isotope labelled quantitative cross-linking approach, we were able to quantify differences in protein-p...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-04-27 | PXD017620 | Pride
Carbon source Cross-linking Mass spectrometry Quantitative Yeast Saccharomyces cerevisiae Qxl-ms Xl-ms Mitochondria 
ProteomicsGTPase driven maturation of the human mitoribosomal peptidyl transferase center
We combine genetic perturbation with cryo-electron microscopy (cryo-EM) to establish the mechanism of PTC maturation during human mitoribosome biogenesis. Cryo-EM structures of large mitoribosomal subunit (mtLSU) assembly intermediates purified from GTPBP6-deficient cells reveal that GTPBP5 acts in ...
ORGANISM(S): Homo sapiens (Human) 
2021-05-20 | PXD023502 | Pride
Ptc maturation Gtpbp5 Gtpbp6 Mitochondrial ribosome Ribosome recycling Ribosome assembly Mterf4-nsun4 Mitoribosome Obg-hflx superfamily 
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