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ProteomicsMudPIT analyses of the proteins co-purified with the Interactor of Little Elongation Complex ELL subunit 1 (FLAG-ICE1), WT MED26, and D2G8 NTD-mutant MED26, affinity-purified from HEK293T cell lines
Protein Complexes Purification: We generated 293FRT cell lines that stably express FLAG-tagged ICE1 (Interactor of Little Elongation Complex ELL subunit 1; KIAA0947). We extracted nuclear proteins in the presence of Benzonase to digest nucleic acids, including both DNA and RNA, and purified the lit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-20 | MSV000083465 | MassIVE
TranscriptomicsTranscription profiling of light and carbon signaling interactions in Arabidopsis
A. thaliana seeds of Columbia (Col-0) ecotype were surface-sterilized, plated on designated media and vernalized for 48 hours at 8C. Plants were grown semi-hydroponically under 16-hr-light, 8-hr-dark cycles at a constant temperature of 23C on basal Murashige and Skoog medium supplemented with 2 mM K...
ORGANISM(S): Arabidopsis thaliana 
2004-01-23 | E-MEXP-71 | biostudies-arrayexpress
ProteomicsMass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization
We describe theoretical concepts and explain practical procedures of an electrospray mass spectrometry method, termed “Intact Transmission Epitope Mapping – Thermodynamic Weak-force Order (ITEM-TWO)”, by which can be determined apparent binding energies and dissociation constants of immune complex ...
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse) 
2020-10-29 | PXD021296 | Pride
Protocol Item-two Rnase s Offline esi-ms Troponin i epitope peptide-antitroponin i antibody complex Myoglobin Flag-peptide–antiflag antibody complex 
TranscriptomicsThe Akt-SRPK-SR axis constitutes a major pathway in transducing EGF signaling to regulate alternative splicing in the nucleus
Pre-mRNA splicing is regulated by developmental and environmental cues, but little is known about how specific signals are transduced in mammalian cells to regulate this critical gene expression step. Here, we report massive reprogramming of alternative splicing in response to EGF signaling. By blo...
ORGANISM(S): Homo sapiens 
2012-08-17 | E-GEOD-37731 | biostudies-arrayexpress
ProteomicsA tissue-based draft map of the murine MHC class I immunopeptidome
The array of peptides presented to CD8+ T cells by major histocompatibility complex (MHC) class I molecules is referred to as the MHC class I immunopeptidome. Although the MHC class I immunopeptidome is ubiquitous in mammals and represents a critical component of the adaptive immune system, very lit...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-02-19 | MSV000084980 | MassIVE
ProteomicsDietary- and host-derived metabolites are used by diverse gut bacteria for anaerobic respiration
Targeted GC-MS detection of host and dietary metabolites and modifications by gut bacteria.
ORGANISM(S): Eggerthella Lenta (ncbitaxon:84112) Holdemania Filiformis (ncbitaxon:61171) Sutterella Wadsworthensis (ncbitaxon:40545) 
2023-11-03 | MSV000093291 | MassIVE
MetabolomicsMetabolomic responses to long-term salt stress in related Lotus species (B)
The legume genus Lotus includes glycophytic forage crops and other species adapted to extreme environments, such as saline soils. Understanding salt tolerance mechanisms will contribute to the discovery of new traits which may enhance the breeding efforts towards improved performance of legumes in m...
2015-09-14 | MTBLS15 | MetaboLights
MetabolomicsMetabolomic responses to long-term salt stress in related Lotus species (C)
The legume genus Lotus includes glycophytic forage crops and other species adapted to extreme environments, such as saline soils. Understanding salt tolerance mechanisms will contribute to the discovery of new traits which may enhance the breeding efforts towards improved performance of legumes in m...
2015-09-14 | MTBLS16 | MetaboLights
MetabolomicsMetabolomic responses to long-term salt stress in related Lotus species (A)
The legume genus Lotus includes glycophytic forage crops and other species adapted to extreme environments, such as saline soils. Understanding salt tolerance mechanisms will contribute to the discovery of new traits which may enhance the breeding efforts towards improved performance of legumes in m...
2015-09-14 | MTBLS14 | MetaboLights
ProteomicsHuman EGFR AP-MS project
Mass spectrometry was applied to comprehensively analyze the phosphorylation, ubiquitination, and interactome of wild type and endocytosis-defective EGFR variants before and after internalization in response to EGF or stress.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-02-23 | MSV000079525 | MassIVE
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