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Protein quantification by iTRAQ was carried out according to the method of Lu et al. (2017). Equally mixed peptides were analyzed by Majorbio Biopharm Technology Co., Ltd. The mixed peptides were preseparated via a C18 reversed-phase column and analyzed via liquid chromatography coupled with tandem ...
ORGANISM(S): Taiwanofungus Camphoratus 
2020-03-11 | PXD018004 |

BACKGROUND: Traditional Chinese medicine has used Peucedanum praeruptorum Dunn (Apiaceae) for a long time. Various coumarins, including the significant root constituents Praeruptorin (A-E), are the active constituents of the dried roots of P. praeruptorum. Previous trans...

2024-04-02 | MTBLS8679 | MetaboLights
Human Trisomy 21 (T21), which causes Down Syndrome (DS), is the most common known cause of intellectual disability. However, the molecular basis for DS phenotypic variability remains poorly understood. Here we used SWATH mass spectrometry (SWATH-MS) to quantify protein abundance and protein turnover...
ORGANISM(S): Homo sapiens (Human) 
2017-11-01 | PXD004880 | Pride
Laboratory mice are standardly housed around 23ºC, setting them under chronic cold stress. Metabolic changes of liver in mice housed under thermoneutral, standard and cold temperatures remain unknown. Here we isolated lipid droplets and mitochondria from their livers for comparative proteomic study ...
ORGANISM(S): Mus musculus (Mouse) 
2019-07-02 | PXD014224 | Pride
LIN28 is a conserved RNA binding protein implicated in pluripotency, reprogramming and oncogenesis. Previously shown to act primarily by blocking let-7 microRNA (miRNA) biogenesis, here we elucidate distinct roles of LIN28 regulation via its direct messenger RNA (mRNA) targets. Through cross-linking...
ORGANISM(S): Homo sapiens 
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