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ProteomicsIgG M-protein quantification by PRM in blood of multiple myeloma patient
The amino acid sequence of the M-protein for multiple myeloma is unique compared to the polyclonal antibodies in patients’ blood. This uniqueness is exploited to develop an ultra-sensitive M-protein detection method utilizing mass spectrometry (MS). The method involves the de novo amino acid sequenc...
ORGANISM(S): Homo Sapiens 
2021-07-26 | PXD022980 | panorama
TranscriptomicsM. catarrhalis Gene Expression in the Chinchilla Nasopharynx
M. catarrhalis strain O35E.rpsl was inoculated into the nasopharynx of healthy, male adult chinchillas. 24 hours later the nasopharyngeal tissues were extracted and homogenized. Total RNA was extracted from these tissue samples. Subsequently, M. catarrhalis genome directed primers were utilized t...
ORGANISM(S): Moraxella catarrhalis 
2012-04-03 | E-GEOD-13559 | biostudies-arrayexpress
TranscriptomicsHigh salt primes a specific activation state of macrophages, M(Na)
Purpose: Investigate effects of high salt on human macrophage activation Methods: Human monocytes-derived macrophages were treated by additional 51mM NaCl for 24 hours (NaCl groups) or not (Control groups). mRNA profiles were generated by RNA-Seq, in triplicate, using Ion proton（Life tech). qRT–PCR ...
ORGANISM(S): Homo sapiens 
2015-09-23 | E-GEOD-68482 | biostudies-arrayexpress
GenomicsTranscription factor binding in human cells occurs in dense clusters formed around cohesin anchor sites [S-phase/M-phase synchronized cells]
During cell division, transcription factors (TFs) are removed from chromatin twice, during DNA synthesis, and during condensation of chromosomes. How TFs can efficiently find their sites following these stages has been unclear. Here, we have analyzed the binding pattern of expressed TFs in human col...
ORGANISM(S): Homo sapiens 
2013-10-01 | E-GEOD-51290 | biostudies-arrayexpress
ProteomicsProteomic analysis of GM-CSF and M-CSF polarized bone marrow derived macrophages
Bone marrow cells were isolated, primed with M-CSF (M-BMDM) or GM-CSF (GM-BMDM) and cultured for 7 days. The proteomic difference between GM-BMDM and M-BMDM were analyzed to describe the phenotye and function of two types of macrophages.
ORGANISM(S): Mus Musculus 
PXD041180 |
ProteomicsMass spectrometry provides a highly sensitive non-invasive means of sequencing and tracking M-protein levels in blood of multiple myeloma patients
De nove sequencing of NISTmAb and of an M-protein from an multiple myeloma patient, MM2232
ORGANISM(S): Homo sapiens (Human) 
2021-07-16 | PXD022784 | Pride
Multiple myeloma M-protein 
ProteomicsCircular RNA circBNC2 regulates epithelial cell G2-M arrest to prevent fibrotic maladaptive repair
Epithelial cells arrested in G2/M phase of the cell cycle after injury is a key checkpoint for determining the wound-healing to either normal cell proliferation or fibrosis. The mechanism that regulates the fibrogenic response after injury is not well understood. Here, we identified a kidney- and li...
ORGANISM(S): Homo Sapiens 
PXD033284 |
TranscriptomicsTranscriptional profiling of ATCC46645 strain and isogenic M-NM-^TpacC Aspergillus fumigatus mutant during murine infection
In lung diseases caused by the major mould pathogen Aspergillus fumigatus the pulmonary epithelium is destroyed by invasive growth of fungal hyphae, a process thought to require fungal proteases. Here we show that the A. fumigatus pH-responsive transcription factor PacC governs expression of secrete...
ORGANISM(S): Aspergillus fumigatus 
2014-10-20 | E-GEOD-54810 | biostudies-arrayexpress
ProteomicsMass spectrometry runs and analysis of host proteins co-immunoprecipitated with RABV M protein
To explore the role of the matrix protein in RABV infection of host cells, we looked for host proteins that interact with the RABV M protein. We performed Flag-tag-based immunoprecipitation of lysates of HEK293T cells transfected with plasmids expressing either N- or C-terminally Flag-tagged M prote...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD021039 | Pride
Matrix protein Co-immunoprecipitation Host proteins Rabv Mass spectrometry 
TranscriptomicsIdentification of drought stress-responsive transcription factor in ramie (Boehmeria nivea LM-oM-
Drought is a main environment stress which severely inhibits the stem growth of ramie and lead to a decrease of the fiber yield. The mechanisms of ramie responding to drought stress are poorly understood. Using Illumina sequencing, approximately 4.8 million (M) 21-nt cDNA tags were sequenced in the ...
ORGANISM(S): Boehmeria nivea 
2013-04-23 | E-GEOD-46253 | biostudies-arrayexpress
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