Regulation of metabolic enzymes through degradation by the ubiquitin-proteosome system regulation is essential towards maintenance of cellular homeostasis. Our approach directly addresses a problem in the field; linking metabolic signal to an E3 ligase response. We employ active E3 ligase profiling ...
Active cullin-RING profiling and total proteome mass spectrometry were used to uncover the inverse, cysteine-dependent, releationship between the E3 LRRC58 and its substrate CDO1.
Protein regulation of metabolic processes is foundational to biology. Here we develop a mass spectrometry-based approach that leverages genetic diversity to nominate functional relationships between 285 metabolites and 11,868 proteins in living tissues. This Metabolite Protein Covariation Architectu...
Precise control of microRNA (miRNA) expression is critical for normal development. An important mechanism of miRNA regulation is target-directed microRNA degradation (TDMD), a pathway in which the binding of miRNAs to specialized trigger RNAs induces ubiquitylation and decay of associated Argonaute ...
