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ProteomicsTargeted Proteomics of NERD and FLS2 on LTQ-Orbitrap Velos Mass Spectrometer
Targeted proteomics has become increasingly popular recently because of its ability to precisely quantify selected proteins in complex cellular backgrounds. We demonstrate the utility of an LTQ-Orbitrap Velos Pro mass spectrometer in targeted parallel reaction monitoring (PRM) despite its unconventi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-12-09 | PXD001119 | Pride
Ltq-orbitrap Prm Fls2 Targeted proteomics Nerd 
UnknownImmunodepletion plasma proteomics by tripleTOF 5600 and Orbitrap elite/LTQ-Orbitrap Velos/Q exactive mass spectrometers.
Not available
S-EPMC3817719 | biostudies-literature
UnknownComparison of the LTQ-Orbitrap Velos and the Q-Exactive for proteomic analysis of 1-1000 ng RAW 264.7 cell lysate digests.
Not available
S-EPMC3673017 | biostudies-literature
ProteomicsIn depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer
A shotgun proteomic approach was used to obtain comprehensive dataset of cumin proteins. For this purpose we applied a one dimensional electrophoresis coupled with high resolution mass spectrometry. The first step in our proteome analysis strategy, we evaluate different extraction methods, based on ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-28 | PXD006425 | Pride
Plant proteins Cumin Lc-ms/ms 
MetabolomicsCoordinate Regulation of Metabolite Glycosylation and Stress Hormone Biosynthesis by TT8 in Arabidopsis
Secondary metabolites play a key role in coordinating ecology and defense strategies of plants. Diversity of these metabolites arise by conjugation of core structures with diverse chemical moieties, such as sugars in glycosylation. Active pools of phytohormones, including those involved in plant str...
2016-10-03 | MTBLS129 | MetaboLights
ProteomicsAnalysis of mouse hippocampus membrane proteome by gel-based LC/MS
Mouse hippocampus membrane fractions were prepared using sucrose density gradient ultracentrifugation. Membrane proteins were solubilized using three different condition; 6-ACA/Triton X-100 or ProteoExtract Native Membrane Protein Extraction Kit (Calbiochem, Cat. No. 444810) or ProteoExtract Transme...
ORGANISM(S): Mus musculus (Mouse) 
2014-08-15 | PXD000311 | Pride
Mouse hippocampus Ltq orbitrap velos pro Membrane proteins Gel-based proteomics In-gel chymotrypsin/trypsin digestion 
MetabolomicsImpact of collection conditions on the metabolite content of human urine samples as analyzed by liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectroscopy (LC-MS assay)
There is a lack of comprehensive studies documenting the impact of sample collection conditions on metabolic composition of human urine. To address this issue, two experiments were performed at a three-month interval, in which midstream urine samples from healthy individuals were collected, pooled, ...
2014-12-18 | MTBLS148 | MetaboLights
UnknownAccurate label-free protein quantitation with high- and low-resolution mass spectrometers.
Not available
S-EPMC3946283 | biostudies-literature
ProteomicsOptimizing chromatographic conditions for orbitrap-based MS platforms
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
ORGANISM(S): Homo sapiens (Human) 
2019-03-04 | PXD009875 | Pride
Reproducibility Data-dependent acquisition Chromatography Q exactive Fusion lumos Human Pearson product-moment correlation coefficient Distributed normalized spectral abundance factor Orbitrap Quantitative proteomics Multidimensional protein identification technology 
ProteomicsHost response profile of human brain proteome in toxoplasma encephalitis
Frontal cortex brain tissues obtained from 5 toxoplasma encephalitis patients and 5 controls were used for protein extraction. Protein amount was normalized on 10% SDS-PAGE, equal amount of protein from 5 patients and 5 controls were pooled separately and subjected to reduction, alkylation and try...
ORGANISM(S): Homo sapiens (Human) 
2016-05-09 | PXD000261 | Pride
Ltq-orbitrap velos Immunosuppression Neuroinfections 
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