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Cysteine modifications have been widely detected among different protein in different species. Here, we examined protein glutathionylation in HeLa cells under both untreated and diamide-treated conditions. A strategy using resin-assisted enrichment of glutathionylated proteins or peptides after biot...
ORGANISM(S): Homo sapiens (Human) 
2020-04-29 | PXD017717 | Pride
This project provides tandem mass spectrometry datasets of an artificial microbiota composed of 24 microbial strains: Bacillus cereus ATCC 14579, Bacillus subtilis ATCC 6633, Bacillus thuringiensis DSM 5815, Bordetella parapertussis Bpp5, Cellulophaga lytica DSM 7489, Deinococcus deserti VCD115, Dei...
ORGANISM(S): Bordetella parapertussis Bpp5 Deinococcus geothermalis DSM 11300 Marivirga tractuosa DSM 4126 Bacillus subtilis subsp. spizizenii ATCC 6633 Salmonella bongori NCTC 12419 Kineococcus radiotolerans SRS30216 = ATCC BAA-149 Deinococcus deserti (strain VCD115 / DSM 17065 / LMG 22923) Shigella flexneri 2a str. 2457T Pseudomonas putida KT2440 Sagittula stellata E-37 Vibrio harveyi NBRC 15634 = ATCC 14126 Staphylococcus carnosus subsp. carnosus TM300 Bacillus cereus (strain ATCC 14579 / DSM 31) Phaeobacter gallaeciensis (strain ATCC 700781 / DSM 17395 / CIP 105210 / NBRC 16654 / BS107) (Roseobacter gallaeciensis) Oceanibulbus indolifex HEL-45 Bacillus thuringiensis serovar berliner ATCC 10792 Oceanicola granulosus HTCC2516 Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) Pseudopedobacter saltans DSM 12145 Ruegeria pomeroyi DSS-3 Cellulophaga lytica DSM 7489 Deinococcus proteolyticus MRP Roseovarius nubinhibens ISM Sphingomonas wittichii RW1 
2023-06-02 | PXD005759 | Pride
26S proteasome was affinity-purified with anti-FLAG beads and separated by 2-DE method. Each spot was cut and digested by Trypsin enzyme, which finaly applied for LC-MS/MS nalysis using a LTQ-Orbitrap XL (Thermo Scientific). The obtained spectra were compared against 35,386 sequences in The Arabidop...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2014-07-28 | PXD000417 | Pride
Global ageing poses a substantial economic burden on health and social care costs. Enabling a greater proportion of older people to stay healthy for longer is key to the future sustainability of health, social and economic policy. Frailty and associated decrease in resilience plays a central role in...
2019-09-06 | MTBLS598 | MetaboLights
In the current study, we used a mass spectrometry-based redox proteomics approach to test responses of the cysteine (Cys) proteome to selective disruption of the Trx- and GSH-dependent systems. Auranofin (ARF) was used to inhibit Trx reductase without detectable oxidation of the GSH/GSSG couple, and...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD000353 | Pride
Neuropathic pain is a major clinic probelm as it is very difficult to treat and mechanism remain unknown. Here, we investigated the differential expression of proteins in the central nuecleus of amygdala (CeA) in neuropathic pain moldel spinal nerve transection (SNT)in rats. CeA was excised from nai...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-02-21 | PXD017473 | Pride
The age of mosquitoes is a crucial determinant of their susceptibility to infection, probability of survival to transmit pathogens and tolerance to insecticides. We investigated changes to the abundance of proteins found in heads and thoraces of Anopheles gambiae and Anopheles stephensi as they aged...
ORGANISM(S): Anopheles gambiae (African malaria mosquito) Anopheles stephensi (Asian malaria mosquito) 
2015-08-26 | PXD002153 | Pride
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