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ProteomicsSynechocystis sp. PCC 6803 proteome response to P-deficiency
Lipid droplets (LDs) are well integrated into multiple facets of cellular physiology and potentially represent an effective platform for the engineering of heterologous metabolic pathways. LDs of higher plant chloroplasts, known as plastoglobules (PGs), are critical mediators of stress tolerance thr...
ORGANISM(S): Synechocystis sp. PCC 6803 
2025-05-21 | PXD051903 | Pride
Cyanobacteria Cyanoglobules Phosphorous Phosphate 
ProteomicsComparison of wild type and abc1k6-1 mutant total leaf extracts from 4 and 5 week old plants
The quantitative proteomes of leaf tissue from A. thaliana at 4 weeks and 5 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA insertion mutant disrupting the plastoglob...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030750 | Pride
Coq8 Abc1k6 Leaf 
ProteomicsQuantitative proteomics of isolated A. thaliana plastoglobules from wild-type, abc1k6-1 and Comp3
The quantitative proteomes of isolated plastoglobules have been determined from A. thaliana leaf chloroplasts at 4 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA ins...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030698 | Pride
Coq8 Plastoglobules Adck3 Abc1 
Proteomicscoimmunoprecipitation of BdRFS identifies putative itneracting proteins
In this work we sought to identify putative interacting proteins of the Brachypodium distachyon Regulator of Stress and Flowering protein (BdRFS) from healthy leaf tissue. A truncated form of BdRFS lacking the N-terminal 45 residues was necessary for solubilization. THe protein is tagged N-terminall...
ORGANISM(S): Brachypodium distachyon (Purple false brome) (Trachynia distachya) 
2023-03-11 | PXD033835 | Pride
Rfs Brachypodium 
ProteomicsHeterologous Expression of MBP-AtABC1K6-His in E. coli
Heterologous E. coli expression of AtABC1K6 tagged with maltose binding protein (MBP) and 6xHis tags revealed a spontaneous cleavage event of the recombinant protein during purification. Resulting bands were resolved on SDS-PAGE and excised for MS/MS identification. Mass spec-based identification of...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030384 | Pride
Coq8 Kinase Spontaneous cleavage Ubib Abc1 
ProteomicsB73 maize thylakoid proteome under a heat stress time-course
A heat stress treatment was imposed on B73 inbred maize three weeks after germination by increasing ambient air temperatures in the greenhouse from 25C to 30C for one day followed by 40C for 7 days, before a recovery phase at 25C for 3 days. We developed a broad-level view of heat stress effects by ...
ORGANISM(S): Zea mays (Maize) 
2025-10-17 | PXD061472 | Pride
Heat Thylakoid Maize 
ProteomicsB73 maize plastoglobule proteome under a heat stress time-course
A heat stress treatment was imposed on B73 inbred maize three weeks after germination by increasing ambient air temperatures in the greenhouse from 25C to 30C for one day followed by 40C for 7 days, before a recovery phase at 25C for 3 days. We developed a broad-level view of heat stress effects by ...
ORGANISM(S): Zea mays (Maize) 
2025-10-17 | PXD061473 | Pride
Plastoglobule Heat Maize 
ProteomicsB73 maize total leaf proteome under drought
A water-deficit treatment was imposed on B73 inbred maize three weeks after germination by withholding water. We developed a broad-level view of water-deficit effects by determining the quantitative total leaf proteome at the reference unstressed time-point (7dWW) and the peak water-deficit stress t...
ORGANISM(S): Zea mays (Maize) 
2025-10-17 | PXD057023 | Pride
Maize 
ProteomicsB73 maize total leaf proteome under a heat stress time-course
A heat stress treatment was imposed on B73 inbred maize three weeks after germination by increasing ambient air temperatures in the greenhouse from 25C to 30C for one day followed by 40C for 7 days, before a recovery phase at 25C for 3 days. We developed a broad-level view of heat stress effects by ...
ORGANISM(S): Zea mays (Maize) 
2025-10-17 | PXD061470 | Pride
Heat Leaf Maize 
ProteomicsShotgun Proteomics of BN-PAGE-Resolved Chloroplasts
Blue Native-PAGE resolves protein complexes in their native state. When coupled with immunoblotting, it can be used to identify the presence of high molecular weight complexes at high resolution for any protein, given a suitable antibody. To identify proteins in high molecular weight complexes on a ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-23 | PXD003162 | Pride
Oligomeric state Native gel Protein complex Native molecular weight Chloroplast 
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