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RAD18 and RAD6 were co-expressed in E.coli, RAD18 harbors an N-terminal 6His tag which was utilized for affinity chromatography via cobalt affinity resin. The complex was then subjected to size exclusion chromatography. 5 X molar excess MAGEA4 (also has N-terminal His-tag) was added to RAD18-RAD6. T...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047929 | Pride
Bone is the most frequent site of metastasis in prostate cancer (PCa) and patients with bone metastases are deemed incurable. Targeting prostate cancer cells that disseminated to the bone marrow (BM) prior to surgery and before metastatic outgrowth may therefore prevent lethal metastasis. This promp...
ORGANISM(S): Homo sapiens 
Auto-ubiquitination assay performed in which the E3 ligase complex RAD18-RAD6 was incubated with E1 and ubiquitin in the presence/absence of MAGEA4for 1 hour at 37C. All proteins were purified from E.coli. Samples were loaded and run onto a precast SDS PAGE gel, which was stained with Coomasie. Appr...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047850 | Pride
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