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ProteomicsDiGly_Analysis_RAD18_RAD6_with/without_MAGEA4
Auto-ubiquitination assay performed in which the E3 ligase complex RAD18-RAD6 was incubated with E1 and ubiquitin in the presence/absence of MAGEA4for 1 hour at 37C. All proteins were purified from E.coli. Samples were loaded and run onto a precast SDS PAGE gel, which was stained with Coomasie. Appr...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047850 | Pride
Digly Rad18 Ubiquitination Magea4 Rad6 
GenomicsRole of Rad18 in glioblastoma proliferation
Role of Rad18 in glioblastoma proliferation
PRJNA1190432 | ENA
TranscriptomicsTranscription profiling of wild type and Rad6 or Rad18 knock-out yeast to identify genes regulated by Rad6/Rad18
To identify the yeast genes whose transcription is regulated by Rad6/Rad18
ORGANISM(S): Saccharomyces cerevisiae 
2008-02-20 | E-MEXP-1463 | biostudies-arrayexpress
TranscriptomicsRole of Rad18 in glioblastoma proliferation
The Rad18 E3 ubiquitin ligase, a key regulator of DNA damage tolerance,also functions in repair of DNA double strand breaks. Rad18 is overexpressed in the brain cancer glioblastoma (GBM) and its downregulation sensitizes GBM cells to DNA damaging agents. Here we show that Rad18 has an essential role...
ORGANISM(S): Homo sapiens 
2025-06-30 | GSE282798 | GEO
ProteomicsClass I HDAC overexpression promotes temozolomide resistance in glioma cells by regulating RAD18 expression
Overexpression of histone deacetylases (HDACs) in cancer commonly causes resistance to genotoxic based therapies. Here we report on the novel mechanism whereby overexpressed class I HDACs increase the resistance of glioblastoma cells to the SN1 methylating agent temozolomide (TMZ). The chemotherapeu...
ORGANISM(S): Homo sapiens (Human) 
2022-04-01 | PXD028683 | Pride
Lc-ms/ms 
UnknownMeiotic functions of RAD18.
Not available
S-EPMC3213229 | biostudies-literature
ProteomicsIPs of SETD1A COMPASS-HUMAN LC-MSMS
Mapping physical interactions of SETD1A COMPOASS in HEK293T cells using endogenous immunoprecipitation followed by label-free quantitative proteomics. We found a novel protein interacts with SETD1A, which is RAD18 (DNA damage repair protein).
ORGANISM(S): Homo sapiens (Human) 
2019-06-13 | PXD012549 | Pride
Lc-msms Human Setd1a compass 
ProteomicsRAD18_RAD6_MAGEA4_Cross-linking_Mass_Spectrometry
RAD18 and RAD6 were co-expressed in E.coli, RAD18 harbors an N-terminal 6His tag which was utilized for affinity chromatography via cobalt affinity resin. The complex was then subjected to size exclusion chromatography. 5 X molar excess MAGEA4 (also has N-terminal His-tag) was added to RAD18-RAD6. T...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047929 | Pride
Magea4 Xlms Rad6 Rad18 
GenomicsREV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis
REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis
PRJNA760305 | ENA
UnknownDNA repair protein Rad18 restricts LINE-1 mobility.
Not available
S-EPMC6203705 | biostudies-literature
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