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Raw mass spec. data files, converted peak lists and TPP results files for KCMF1 links RAD6 to UBR4 and lysosome-mediated degradation by Hong et. al, MCP 2014. RAD6 is a ubiquitin E2 protein with roles in a number of different biological processes. Here, using affinity purification coupled with mass ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-01-01 | MSV000078983 | MassIVE
Protein ubiquitination is an essential process that rapidly regulates protein synthesis, function, and fate in dynamic environments. Among its non-proteolytic functions, K63 ubiquitin accumulates in yeast cells exposed to oxidative stress, stalling ribosomes at elongation. K63 ubiquitin conjugates a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-12 | PXD025727 | Pride
Protein ubiquitination is an essential process that rapidly regulates protein synthesis, function, and fate in dynamic environments. Among its non-proteolytic functions, K63 ubiquitin accumulates in yeast cells exposed to oxidative stress, stalling ribosomes at elongation. K63 ubiquitin conjugates a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-12 | PXD025726 | Pride
To identify the yeast genes whose transcription is regulated by Rad6/Rad18
ORGANISM(S): Saccharomyces cerevisiae 
Effect of Rad6 backside beta-turn mutations on gene expression
Chromatin recruitment of Rad6 determines H3K4me3 in Saccharomyces cerevisiae
The ubiquitin conjugase Rad6 mediates ribosome pausing during oxidative stress
Raw mass spec. data files for KCMF1 links RAD6 to vesicle dynamics by Hong et. al. RAD6 is a ubiquitin E2 protein with roles in a number of different biological processes. Here, using affinity purification coupled with mass spectrometry, we identify a number of new RAD6 binding partners, including t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2014-06-11 | MSV000078734 | MassIVE
Auto-ubiquitination assay performed in which the E3 ligase complex RAD18-RAD6 was incubated with E1 and ubiquitin in the presence/absence of MAGEA4for 1 hour at 37C. All proteins were purified from E.coli. Samples were loaded and run onto a precast SDS PAGE gel, which was stained with Coomasie. Appr...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047850 | Pride
Rad6 E2 ubiquitin-conjugating enzyme and Bre1 E3 ubiquitin ligase catalyze histone H2B Lysine-123 monoubiquitination (H2Bub1), which stabilizes nucleosomes and regulates the trans-histone H3K4 and K79 methylation during gene transcription and other nuclear processes. The interaction interfaces withi...
ORGANISM(S): Saccharomyces cerevisiae 
2022-10-24 | GSE139632 | GEO
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