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ProteomicsBiosynthesis of novel non-proteinogenic amino acids β-hydroxyenduracididine and β-methylphenylalanine in Escherichia coli
Non-proteinogenic amino Q7 Q8 acids (npAAs) are valuable building blocks for the development of advanced pharmaceuticals and agrochemicals. The surge in interest in their synthesis is primarily due to the potential to enhance and diversify existing bioactive molecules. This can be achieved by alteri...
ORGANISM(S): Escherichia coli 
2024-10-01 | PXD056235 | Pride
E. coli Heterologous expression Enduracididine Non-proteinogenic amino acids Streptomyces Β-methylphenylalanine Β- hydroxyenduracididine 
ProteomicsA TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens
Acetogens can fix carbon (CO or CO2) into acetyl-CoA via the Wood-Ljungdahl pathway (WLP) that also makes them attractive cell factories for the production of fuels and chemicals from waste feedstocks. Although most biochemical details of the WLP are well understood and systems-level characterisatio...
ORGANISM(S): Clostridium autoethanogenum 
2019-11-20 | PXD014421 | Pride
Biological  data-dependent acquisition  gas fermentation  acetogen 
ProteomicsThe rapid manufacture of biologics using mRNA
We evaluate bevacizumab, a monoclonal antibody, expression in time course from mRNA transient transfection in CHO cells using MS. We transiently transfected bevacizumab heavy and light chain mRNAs into CHO-S WT using lipoplex. Later, we analysed the bevacizumab protein intracellularly.
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-12-22 | PXD067559 | Pride
Cho Ms Protein expression Monoclonal antibody Mrna transien transfection 
ProteomicsRedox controls metabolic robustness in the gas-fermenting acetogen Clostridium autoethanogenum
Living biological systems display a fascinating ability to self-organise their metabolism. This ability ultimately determines metabolic robustness that is fundamental to control cellular behaviour. However, fluctuations in metabolism can affect cellular homeostasis through transient oscillations. Fo...
ORGANISM(S): Clostridium autoethanogenum 
2020-05-18 | PXD016381 | Pride
Metabolic robustness  oscillations  acetogen  gas fermentation  wood-ljungdahl pathway  metabolism  data-independent acquisition 
ProteomicsH2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum
Background: The global demand for affordable carbon has never been stronger, and there is an imperative in many industrial processes to use waste streams to make products. Gas-fermenting acetogens offer a potential solution and several commercial gas fermentation plants are currently under construct...
ORGANISM(S): Clostridium autoethanogenum 
2018-03-05 | PXD008367 | Pride
Acetogen  gas fermentation  quantitative proteomics  data-independent acquisition  genome-scale modelling  metabolomics 
ProteomicsMulti-omic characterisation of Streptomyces hygroscopicus NRRL 30439: detailed assessment of its secondary metabolic potential
Time course intracellular proteomics of Streptomyces hygroscopicus NRRL 30439 (S. inhibens NRRL30439, CP084681) during submerged culture fermentation. Label free quantification of LC-MSMS spectra was used for protein expression profiling to support primary and secondary metabolic functionalities.
ORGANISM(S): Streptomyces inhibens 
2025-06-03 | PXD030257 | Pride
Intracellular Secondary metabolism Streptomyces Hygroscopicus Inhibens Timecourse 
ModelsCasini2022 - genome scale metabolic model (iMTD22IC) of Methanothermobacter thermautotrophicus Delta H
(iMTD22IC) This model is described in these articles: Casini, I., McCubbin, T., Esquivel-Elizondo, S., Luque, G.G., Evseeva, D., Fink, C., Beblawy, S., Youngblut, N.D., Aristilde, L., Huson, D., Drager, A., Ley, R., Marcellin, E., Angenent, L.T., Molitor, B. 2023. An integrated systems biology app...
2026-03-15 | MODEL2211290001 | BioModels
FROG 
ModelsCasini2022 - Methanothermobacter marburgensis Marburg
This model is described in these articles: Casini, I., McCubbin, T., Esquivel-Elizondo, S., Luque, G.G., Evseeva, D., Fink, C., Beblawy, S., Youngblut, N.D., Aristilde, L., Huson, D., Drager, A., Ley, R., Marcellin, E., Angenent, L.T., Molitor, B. 2023. An integrated systems biology approach reveal...
2026-03-15 | MODEL2211290003 | BioModels
FROG 
ModelsCasini2022 - Methanothermobacter thermautotrophicus Z-245
(iMTD22IC) This model is described in these articles: Casini, I., McCubbin, T., Esquivel-Elizondo, S., Luque, G.G., Evseeva, D., Fink, C., Beblawy, S., Youngblut, N.D., Aristilde, L., Huson, D., Drager, A., Ley, R., Marcellin, E., Angenent, L.T., Molitor, B. 2023. An integrated systems biology ap...
2026-03-15 | MODEL2211290002 | BioModels
FROG 
TranscriptomicsNext Generation Sequencing Analysis of Saccharopolyspora erytharea transcriptomes
We report the high-throughput profiling of saccharopolyspora erythraea life cycle in bioreactors. RNA was harvested at seven time-points from bioreactors, three points immediately before, one during and three following the metabolic switch (as evidenced by biomass cell dry weight). We prepared unstr...
ORGANISM(S): Saccharopolyspora erythraea 
2013-06-18 | E-GEOD-39722 | biostudies-arrayexpress
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