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ProteomicsA screen of Campylobacter jejuni protein microarrays with antisera to identify immunogenic proteins
Campylobacter jejuni (C. jejuni) protein microarrays were used to identify immunogenic C. jejuni proteins that may be useful in the development of biomarkers, diagnostic assays, or subunit vaccines for humans or livestock. A native protein microarray with over 1400 individually purified GST-tagged C...
ORGANISM(S): Mus musculus 
2018-12-29 | E-MTAB-7535 | biostudies-arrayexpress
MetabolomicsThe patatin-like protein PlpD forms novel structurally dynamic homodimers in the Pseudomonas aeruginosa outer membrane

Members of the Omp85 superfamily of outer membrane proteins (OMPs) found in Gram-negative bacteria, mitochondria and chloroplasts are characterized by a distinctive 16-stranded β-barrel transmembrane domain and at least one periplasmic POTRA domain. All previously studied Omp85 proteins promote c...

2025-12-08 | MTBLS9803 | MetaboLights
ProteomicsDiscovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches
A key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic food protein analysis methods is to use protein marker peptides with good analytical performances in LC-MS analysis of commercial processed foods. In this study, we developed a multistage walnut protein marker pept...
ORGANISM(S): Juglans regia (English walnut) 
2023-07-31 | PXD043010 | Pride
Food allergy Lc-ms Validation Walnut marker peptide 
ProteomicsDetection of immunogenic proteins from bacteria
Detection of immunogenic proteins remains an important task for life sciences as it nourishes the understanding of pathogenicity, illuminates new potential vaccine candidates and broadens the spectrum of biomarkers applicable in diagnostic tools. Traditionally, immunoscreenings of expression librari...
ORGANISM(S): Campylobacter jejuni 
2011-10-26 | E-GEOD-33295 | biostudies-arrayexpress
TranscriptomicsGlioblastoma microvesicles transport RNA and proteins
Glioblastoma tumour cells release microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by normal host cells, such as brain microvascular endothelial cells. By incorporating an mRNA for a reporter protein into these microvesicles, we demonstrate th...
ORGANISM(S): Homo sapiens 
2008-11-15 | E-GEOD-13470 | biostudies-arrayexpress
ProteomicsProfiling of antibodies against prostate cancer xenograft-released proteins
The present study describes a novel xenograft-based biomarker discovery platform and proves its usefulness in the discovery of novel serum markers for prostate cancer (PCa). By immunizing immuno-competent mice with serum from nude mice bearing PCa xenografts, an antibody response against xenograft-d...
ORGANISM(S): Mus musculus 
2011-12-16 | E-GEOD-16220 | biostudies-arrayexpress
ProteomicsEvaluation of auto-antibody serum biomarkers for breast cancer screening
Using protein microarrays, derived from 642 His-tag proteins, we could distinguish sera from breast-nodule positive patients and healthy control individuals. Each Protein microarray was divided in to 4 sub-arrays. Each protein was spotted in duplicates in each sub-array. For evaluation 24 malignant,...
ORGANISM(S): Homo sapiens 
2011-12-21 | E-GEOD-34555 | biostudies-arrayexpress
ProteomicsIdentification of universally applicable and species-specific marker peptides for Bacillus anthracis
Anthrax is a zoonotic infection caused by the bacterium Bacillus anthracis (BA), a gram-positive, aerobic, spore-forming bacterium that can be misused as a biowarfare agent. The major patho-genicity factors of BA are encoded by genes located on two extrachromosomal plasmids, which are often targeted...
ORGANISM(S): Bacillus anthracis Bacteria 
2023-01-05 | PXD036243 | Pride
Bacillus anthracis Anthrax Species-specific marker peptides High-resolution mass spectrometry 
ProteomicsCharacterization of complement C3 as a marker of alpha-amanitin toxicity by comparative secretome profiling
In the human body, proteins secreted into peripheral blood vessels are known as the secretome, and they represent the physiological or pathological status of cells. The unique response of cells to toxin exposure can be confirmed via secretome analysis, which can be used to discover toxic mechanisms ...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-23 | PXD038016 | Pride
Alpha-amanitin  toxic mushroom  secretomes  exposure marker  complement component 3 
TranscriptomicsTranscription profiling of human normal and transformed buccal keratinocytes
Normal and two transformed buccal keratinocyte lines were cultured under a standardized condition to explore mechanisms of carcinogenesis and tumor marker expression at transcript and protein level. An approach combining three bioinformatic programs allowed coupling of abundant proteins and large-sc...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7862 | biostudies-arrayexpress
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