Sort   by:  
 Page size 
TranscriptomicsHistone Demethylase-Assisted Somatic Cell Nuclear Transfer Facilitates Derivation of Human Pluripotent Stem Cells
The extremely low efficiency of human embryonic stem cell (hESC) derivation using somatic cell nuclear transfer (SCNT) limits potential application. Blastocyst formation from human SCNT embryos occurs at a low rate and with only some oocyte donors. We previously showed in mice that reduction of hist...
ORGANISM(S): Homo sapiens 
2016-03-01 | E-GEOD-73362 | biostudies-arrayexpress
TranscriptomicsRNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryo
Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient. Most SCNT-generated embryos die after implantation because of unidentified, complex epigenetic errors in the process of postimplantation embryonic development. Here, we identified the most upstream level of dysfunction le...
ORGANISM(S): Mus musculus 
2011-11-17 | E-GEOD-33208 | biostudies-arrayexpress
TranscriptomicsComparative gene expression analyses using E4.0 cloned blastocysts
The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression p...
ORGANISM(S): Mus musculus 
2013-09-01 | E-GEOD-49172 | biostudies-arrayexpress
TranscriptomicsComparative gene expression analyses using post-implanted E6.5 cloned embryos
The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression p...
ORGANISM(S): Mus musculus 
2013-09-01 | E-GEOD-49171 | biostudies-arrayexpress
TranscriptomicsImpeding Xist expression from the active X chromosome improves mouse somatic cell cloning
Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient because of aberrant genomic reprogramming. In addition to random reprogramming errors, we hypothesized the presence of specific errors as evidenced by common anomalies among clones. We found that Xist, which normally inact...
ORGANISM(S): Mus musculus 
2010-09-16 | E-GEOD-23181 | biostudies-arrayexpress
ProteomicsProSeAM SILAC MS screening for CARNMT1 substrates
Click chemistry based substrates screening for CARNMT1 in Carnmt1 KO HEK293T cells.
ORGANISM(S): Homo Sapiens (human) 
2023-08-24 | PXD037965 | JPOST Repository
TranscriptomicsGeneration of Cloned Mice from Adult Neurons by Direct Nuclear Transfer
Whereas cloning mammals by direct somatic cell nuclear transfer has been successful using a wide range of donor cell types, neurons from adult brain remain M-bM-^@M-^\unclonableM-bM-^@M-^] for unknown reasons. Here we examined whether neurons from adult mice could be cloned, using a combination of t...
ORGANISM(S): Mus musculus 
2015-02-03 | E-GEOD-65497 | biostudies-arrayexpress
TranscriptomicsEpigenetic regulation of sex determination by the histone demethylase Jmjd1a
Developmental gene expression is defined through cross-talk between the function of transcription factors and epigenetic status including histone modification. Although several known transcription factors play crucial roles in mammalian sex determination, how chromatin regulation contributes to t...
ORGANISM(S): Mus musculus 
2013-08-03 | E-GEOD-49513 | biostudies-arrayexpress
Sort   by:  
 Page size