Mast cells produce a large amount of several chemokines after cross-linking of FceRI and participate in the pathogenesis of allergic diseases. The objective of this study was to comprehensively investigate FceRI-mediated chemokine induction in human mast cells and the effect of a corticosteroid (dex...
Fucci-expressing HCT116 were inoculated into subcutaneous tissue or cecum of NOD/SCID mice. Four weeks after inoculation, Fucci green (mAG; S/G2/M) and red (mKO2;G1) expressing cells were isolated from implanted tumors by FACSAria sorting (BD, Biosciences). Comparative analyses among green and red c...
Purine catabolism is regarded as a housekeeping function that remobilizes nitrogen for plant growth and development. However, emerging evidence suggests that certain purine metabolites might contribute to stress protection of plants. Here, we show that in Arabidopsis, the intermediary metabolite all...
Calcium signaling plays a pivotal role in t cell activation through activation of kinases and phosphatases, but comprehensive analysis to overview calcium-induced phosphorylation has never been performed. To investigate Ca2+-mediated phosphorylation globally, we next undertook a phosphoproteomic ana...
To define the physiological properties of TCR signaling, We investigated the global dynamic view of TCR-dependent phosphorylation with the use of thymocytes stimulated with antibodies to CD3 to mimic TCR activation
MAPK-Erk and calcium-Calcineurin pathways are major signaling pathways activated during TCR signal transduction. We investigated the effect of these signaling pathway with the use of selective inhibitors of Mek and calcineurin.
To evalute our phospho-iTRAQ system, which combined with IMAC and iTRAQ, with respect to the efficiency of concentration of phosphorylation and quantitation accuracy
We performed transcutaneous electrode stimulation to the tibialis anterior (TA) muscle of rats to mimic resistance exercise and mass spectrometer to measure the changes in protein expression following exercise. Wistar male rats (11-week-old) reared at room temperature (21-25°C) on a 12:12 h light-da...
Mouse oocytes and embryos from the germinal vesicle (GV) stage to the blastocyst stage were collected and treated with acidic Tyrode’s solution to remove the zona pellucida. We used 42.5 oocytes or embryos per sample at each developmental stage and identified the proteome during mouse pre-implantati...
