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ProteomicsDefining the molecular mechanism of PI4KA complex regulation by the phosphatase Calcineurin
To examine the interaction between PI4KIIIa and Calcineurin, HDX-MS experiments comparing calcineurin or PI4KA FAM delta C alone to the calcineurin and PI4KA FAM delta C complex were carried out.
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD043409 | Pride
Hdx-ms Pi4k Calcineurin 
ProteomicsHDX-MS analysis of PI4Ka trimer and FAM126a (415-521) with calcineurin
We used HDX-MS to map novel binding sites of calcineurin on PI4KA and FAM126A. Calcineurin binds to PxIxIT and LxVP motifs on PI4KA and FAM126A indicating a novel regulatory mechanism of Pi4KA by calcineurin
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD050633 | Pride
Hdx-ms Pi4ka Fam126a Calcineurin 
ProteomicsHydrogen Deuterium Exchange of PI4K(530-560) Peptide with Calcineurin
we used HDXMS to study if PI4KA(530-560) is able to bind calcineurin. Here we showed a significant protection in deuterium exchange upon binding calcineurin in the wild type state, indicating these proteins are interacting. Using a mutant that should inhibit the interaction (AKASAA), we showed that ...
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD044636 | Pride
Hdxms Pi4k Calcineurin 
ProteomicsHDXMS of PI4KIIIa and Calcineurin
Using HDX-MS to reveal the binding sites of Calcineurin and PI4KIIIa
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025900 | Pride
Hdx-ms Pi4kiiia Calcineurin 
TranscriptomicsGenome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae
In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a com...
ORGANISM(S): Saccharomyces cerevisiae 
2005-10-15 | E-GEOD-3456 | biostudies-arrayexpress
TranscriptomicsMouse heart tissue: wild-type vs. aMHC-Calcineurin transgenic (CnTg)
Cardiac profiling of miR expression levels in a transgenic mouse model of heart failure (MHC-CnA) to identify miRs that are co-regulated with the development of calcineurin-induced heart failure. Two-condition experiment: WT vs CnTg. Biological replicates: one array was performed on 3 pooled samples...
ORGANISM(S): Mus musculus 
2011-11-02 | E-GEOD-25006 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of primary rat hippocampal culture cells overexpressing an activated form of calcineurin
Micorarray analysis was performed on RNA samples from hippocampal cultures infected with either Ad-aCaN or Ad-LacZ or uninfected. Each sample was applied to its own GeneChip (Rat RG-U34A; n=7-9 chips/group). Chips were then processed and scanned using Agilent Affymetrix GeneArray Scanner. MAS5 was u...
ORGANISM(S): Rattus norvegicus 
2007-09-15 | E-GEOD-1978 | biostudies-arrayexpress
TranscriptomicsDe novo kidney transplantation without use of calcineurin inhibitors preserves renal structure and function
Background: Despite significant improvements in short-term kidney transplant survival, comparable increases in 5 and 10-year outcomes have not been achieved. Chronic allograft nephropathy (CAN) is a major cause of late graft loss. Toxic nephropathy and inadequate long-term immunosuppression are poss...
ORGANISM(S): Homo sapiens 
2004-09-23 | E-GEOD-1743 | biostudies-arrayexpress
TranscriptomicsCalcineurin-dependent transcriptome in ICN1 (activated NOTCH1)-induced T cell acute lymphoblastic leukemia (T-ALL)
Activated NOTCH1 induces T-ALL in mice when transduced in bone marrow (BM) cells. T-ALL cells activate the calcineurin/NFAT pathway in vivo (Medyouf H. et al. Nat Med 2007 [PMID 17515895]). Through appropriate crosses, mouse ICN1-induced T-ALL were generated that include a ROSCreERT2 transgene and 2...
ORGANISM(S): Mus musculus 
2014-08-09 | E-GEOD-60258 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with calcium, sodium
In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a com...
ORGANISM(S): Saccharomyces cerevisiae 
2005-09-23 | E-SMDB-1427 | biostudies-arrayexpress
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