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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
ProteomicsDiscovery of Regulatory Complexes in Arabidopsis via Protein–Metabolite Mapping During Pseudomonas Infection
Protein–metabolite interactions (PMIs) are critical regulators of cellular processes, yet their roles in plant–pathogen interactions remain poorly understood. Here, we applied PROMIS (Protein–Metabolite Interactions using Size separation) to map the Arabidopsis interactome during infection with Pseu...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Pseudomonas syringae pv. tomato str. DC3000 Bacteria 
2026-05-05 | PXD069625 | Pride
Nata1 Promis Protein-metabolite interactions 
MetabolomicsTranscriptomic and Metabolomics Profiling of Phage–Host Interactions between Phage PaP1 and Pseudomonas aeruginosa
The basic biology of bacteriophage–host interactions has attracted increasing attention due to a renewed interest in the therapeutic potential of bacteriophages. In addition, knowledge of the host pathways inhibited by phage may provide clues to novel drug targets. However, the effect of phage on ba...
2018-05-03 | MTBLS431 | MetaboLights
ProteomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Herein, we describe a method, dubbed PROMIS that allows simultaneous, global analysis of endogenous protein–small-molecule and protein-protein complexes. To this end a cell-free native lysate was subjected to size-exclusion chromatography followed by quantitative metabolomic and proteomic analysis. ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-06-06 | PXD009714 | Pride
Protein-metabolite Size exclusion chromatografy Deconvolution Correlation Interactions 
MetabolomicsMulti-kingdom profiling reveals altered gut phage-bacteria-metabolite interactions in MASLD

Metabolic dysfunction-associated steatotic liver disease (MASLD) is increasingly linked to gut microbial dysbiosis. To date, most studies have been limited to bacterial taxa, neglecting viruses and fungi, and their interactions. Here we performed shotgun metagenomic and ITS2 sequencing on fecal s...

2026-03-18 | MTBLS14073 | MetaboLights
ProteomicsProteometabolomics for unsupervised identification of protein–metabolite interactions
Protein–metabolite interactions play an important role in the cell’s metabolism and many methods have been developed to screen them in vitro. However, few methods can be applied at a large scale and not alter biological state. Here we describe a proteometabolomic approach, using chromatography to ge...
ORGANISM(S): Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 
2021-04-27 | PXD025571 | Pride
Protein–metabolite interactions Lc-ms/ms 
MetabolomicsA Murine Model of Maternal Micronutrient Deficiencies and Gut Inflammatory Host-microbe Interactions in the Offspring.

BACKGROUND & AIMS: Micronutrient deficiency (MND) (ie, lack of vitamins and minerals) during pregnancy is a major public health concern. Historically, studies have considered micronutrients in isolation; however, MNDs rarely occur alone. The impact of co-occurring MNDs on pu...

2024-10-18 | MTBLS3476 | MetaboLights
ProteomicsDynamic maps of protein-protein-metabolite complexes in S. cerevisiae
The project aims to create dynamic maps of protein-protein-metabolite complexes in S. cerevisiae across growth phases using PROMIS (PROtein–Metabolite Interactions using Size separation). It is a biochemical, untargeted, proteome- and metabolome-wide method to study protein-protein and protein–metab...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-25 | PXD026639 | Pride
Protein-metabolite complexes Ligand Protein-protein complexes 
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