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This study presents a comparative study of different methodologies for the extraction of proteins from platelet derived-extracellular vesicles (pEV) prior to subsequent mass spectrometry (MS) analysis. Protein isolation is performed through three different methodologies based on SDS-polyacrylamide g...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD060357 | Pride
Transcriptomics data obtained from limiting amounts of mRNA is often noisy, providing primarily qualitative changes in transcript expressions. So far, technical variations arising out of the library preparation protocols have not been adequately characterized at reduced levels of mRNA. Here, we gene...
ORGANISM(S): Mus musculus 
Various Methodologies of Microbiota Assessment for Evaluation of Fecal Microbiota Transplantation and for Super-Donor Recognition Gives More Data Than Each Method Separately
A study to evaluate techniques for repairing DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples by direct comparison of FFPE DNA repair methods prior to analysis on genome-wide methylation array to matched fresh frozen (FF) tissues. Formalin-fixed paraffin-embedded tissue from three col...
ORGANISM(S): Homo sapiens 
Mechanistic study of H2S interference on swine, we used TMT-based comparative proteomics of lung tissues from control and hydrogen sulfide (H2S) breathing swines as a test case for evaluation of MS2, SPS MS3 and RTS-MS3 acquisition methods in both Orbitrap Fusion and Orbitrap Eclipse.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2023-06-02 | PXD020392 | Pride
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