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ProteomicsVacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.
Nano-flow chromatography-tandem mass spectrometer (nLC-MS/MS) is a popular choice in proteome research as it provided high-sensitivity analysis with minimal sample usage, but its quantitative proteomics applications become restricted by low analytical throughput, and low robustness. To circumvent th...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-05-24 | PXD040497 | Pride
Slice-pasef Electrospray ionization (esi) Dia-pasef Micro-flow rate chromatography Vacuum insulated probe heated electrospray ionization source (vip-hesi) Captive spray (cs) 
ProteomicsRobust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 µl/min f...
ORGANISM(S): Homo sapiens (Human) 
2021-02-22 | PXD023650 | Pride
Micro-flow lc Plasma Proteomics Urine 1.0 mm id column 
UnknownVacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.
Not available
S-EPMC9949110 | biostudies-literature
UnknownPortable comprehensive two-dimensional micro-gas chromatography using an integrated flow-restricted pneumatic modulator.
Not available
S-EPMC9622416 | biostudies-literature
UnknownA Micro-Flow Liquid Chromatography-Mass Spectrometry Method for the Quantification of Oxylipins in Volume-Limited Human Plasma.
Not available
S-EPMC11952279 | biostudies-literature
ProteomicsRobust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d.?×?150?mm column and at a flow rate of 50?µl/min f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-27 | MSV000098370 | MassIVE
ProteomicsMicro-flow size-exclusion chromatography for enhanced native mass spectrometry of proteins and protein complexes
Datasets part of manuscript submitted at analytica chimica acta Size-exclusion chromatography (SEC) employing aqueous mobile phases with volatile salts at neutral pH combined with native mass spectrometry (nMS) is a valuable tool to characterize proteins and protein aggregates in their native state...
ORGANISM(S): Reference Proteins 
2023-03-29 | MSV000091582 | MassIVE
ProteomicsImproved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Nano-lc-esi-ms/ms nano-flow hplc hela cell digest 
ProteomicsHigh-throughput proteomics of nanogram-scale samples with Zeno SWATH DIA
The ability to conduct high-quality proteomic experiments in high throughput has opened new avenues in clinical research, drug discovery, and systems biology. Next to an increase in quantitative precision, recent developments in high-throughput proteomics have also gained proteomic depth, to the ext...
ORGANISM(S): Cicer arietinum (Chickpea) (Garbanzo) Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2022-12-01 | PXD036786 | Pride
Dia Zeno swath 
ProteomicsThe Importance of Extracellular Vesicle Purification for Downstream Analysis: A Comparison of Differential Centrifugation and Size Exclusion Chromatography for Parasitic Helminths
Fascioliasis is a major neglected tropical disease that infects humans and ruminant species worldwide. Recent discoveries have identified that liver fluke extracellular vesicles are released into the host’s internal environment. To understand extracellular vesicle biology further, isolation of the e...
ORGANISM(S): Fasciola hepatica (Liver fluke) 
2019-02-13 | PXD008737 | Pride
Extracellular vesicles Size exclusion chromatography and fasciola hepatica. Purification Differential centrifugation 
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