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UnknownSlice-PASEF: Maximising Ion Utilisation in LC-MS Proteomics.
Not available
S-EPMC12424990 | biostudies-literature
ProteomicsSystematic evaluation of PASEF acquisition strategies in complex metaproteomes
Trapped ion mobility spectrometry coupled with parallel accumulation–serial fragmentation (PASEF) has enabled multiple acquisition strategies for proteomics, yet their comparative performance in complex metaproteomes remains unclear. Here we benchmark five modes—DDA-, DIA-, Slice-, Synchro- and midi...
ORGANISM(S): Mus musculus (Mouse) 
2026-07-03 | PXD073688 | Pride
Pasef Metaproteomics 
ProteomicsHigh throughput single-cell proteomics of in-vivo cells
Major advancements in single-cell proteomics (SCP) methodologies have been introduced in recent years, providig highly sensitive sample preparation methods and mass spectrometric technologies. However, most studies present limited throughput and mainly focus on the analysis of cultured cells. To enh...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-07-09 | PXD058457 | Pride
Macrophages Single cell proteomics Fixation Murine Lc-ms Hela Bravo Slice-pasef Timstof Automation 
ProteomicsSystematic evaluation of PASEF acquisition strategies in complex metaproteomes
Trapped ion mobility spectrometry coupled with parallel accumulation–serial fragmentation (PASEF) has enabled multiple acquisition strategies for proteomics, yet their comparative performance in complex metaproteomes remains unclear. Here we benchmark five modes—DDA-, DIA-, Slice-, Synchro- and midi...
ORGANISM(S): Salinibacter ruber DSM 13855 Homo sapiens (Human) Ligilactobacillus murinus DSM 20452 = NBRC 14221 
2026-07-03 | PXD073779 | Pride
ProteomicsVacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.
Nano-flow chromatography-tandem mass spectrometer (nLC-MS/MS) is a popular choice in proteome research as it provided high-sensitivity analysis with minimal sample usage, but its quantitative proteomics applications become restricted by low analytical throughput, and low robustness. To circumvent th...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-05-24 | PXD040497 | Pride
Slice-pasef Electrospray ionization (esi) Dia-pasef Micro-flow rate chromatography Vacuum insulated probe heated electrospray ionization source (vip-hesi) Captive spray (cs) 
UnknownHigh-Throughput Single-Cell Proteomics of In Vivo Cells.
Not available
S-EPMC12301781 | biostudies-literature
UnknownVacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.
Not available
S-EPMC9949110 | biostudies-literature
UnknownVacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer.
Not available
S-EPMC11060334 | biostudies-literature
ProteomicsValidation of Thin-diaPASEF for maximizing proteome coverage
We optimized the parameters of CCS range, polygon scan region, ramp time, and isolation window width to achieve high-depth proteome analysis using the timsTOF HT. As a result, we developed Thin-diaPASEF, which was optimized with a CCS range of 0.7–1.3, a ramp time of 150 ms, an isolation window widt...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2025-08-10 | PXD061613 | JPOST Repository
ProteomicsSpatial Proteomics by Trapped Ion Mobility supported MALDI MS/MS Imaging: A First Glance into Multiplexed and Spatial Peptide Identification
RATIONALE In spatial proteomics, matrix-assisted laser desorption/ionization (MALDI) imaging enables rapid and cost-effective peptide measurement. Yet, in situ peptide identification remains challenging. Therefore, this study aims to integrate the trapped ion mobility spectrometry (TIMS)-based paral...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-11-11 | MSV000096373 | MassIVE
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