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MetabolomicsA microfluidic chip recapitulating the human neurovascular unit with a functional blood-brain barrier for modeling gut-brain interactions

The blood-brain barrier (BBB) is essential for central nervous system homeostasis, but most current in vitro models lack structural and functional fidelity. We developed a physiologically relevant human neurovascular unit microfluidic chip (hNVU-on-a-chip) incorporating brain microvascular endoth...

2025-10-28 | MTBLS13230 | MetaboLights
MetabolomicsSpatiotemporal determination of metabolite activities in the corneal epithelium on a chip
The corneal epithelial barrier maintains the metabolic activities of the ocular surface by regulating membrane transporters and metabolic enzymes responsible for the homeostasis of the eye as well as the pharmacokinetic behavior of drugs. Despite its importance, no established biomimetic in vitro me...
2021-07-13 | MTBLS2274 | MetaboLights
MetabolomicsOrganotypic microfluidic breast cancer model reveals starvation-induced spatial-temporal metabolic adaptations
BACKGROUND: Ductal carcinoma in situ (DCIS) is the earliest stage of breast cancer. During DCIS, tumor cells remain inside the mammary duct, growing under a microenvironment characterized by hypoxia, nutrient starvation, and waste product accumulation; this harsh microenvironment promotes genomic in...
2019-02-08 | MTBLS669 | MetaboLights
MetabolomicsCaco-2-derived spheroids and Organ-on-a-Chip for Food Safety Risk Assessment: Mechanisms of Intestinal Inflammation Induced by 20 nm Polystyrene Nanoplastics
Microplastics released from food packaging have become an emerging food safety concern and may contribute to intestinal inflammation. This study employed Caco-2-derived spheroids combined with self-developed microfluidic organ-on-a-chip technology to systematically investigate the effects of 20 nm p...
2026-10-19 | MTBLS15198 | MetaboLights
ProteomicsMultiphasic chip performance characterization using human thyroid cancer cell line SW1736 and human plasma
Development of an online peptide fractionation system comprising a multiphasic LC chip that integrates reversed phase and strong cation exchange chromatography upstream of the MS based on the multi-dimensional protein identification technology (mudPIT). Chips were assessed in terms of chromatographi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000081545 | MassIVE
OtherOnline peptide fractionation using a multiphasic microfluidic LC chip improves reproducibility and detection limits for quantitation in discovery and targeted proteomics.
Data from ProteomeXchange, PXD ID: PXD001464. Experiment: 1hRP, file: CL_1hRP_rep4-Cell_Lysate_rep4.mgf. Published as part of Mol Cell Proteomics. 2015 Apr 7 . From the Abstract: {{i}} Comprehensive proteomic profiling of biological specimens usually requires multi-dimensional chromatographic pepti...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM11210037376 | GPMDB
Resource Reanalysis 
ProteomicsProteomic reveals reasons for acquired drug resistance in lung cancer derived brain metastasis based on a newly established multi-organ microfluidic chip model
Anti-tumor drugs can effectively shrink the lesions of primary lung cancer; however, it has limited therapeutic effect on patients with brain metastasis (BM). A BM preclinical model based on a multi-organ microfluidic chip has been established proficiently in our previous work. In this study, the BM...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD021760 | Pride
Lung cancer  brain metastasis  microfluidic organ-on-a-chip  drug resistance  proteomic. 
ProteomicsMicroproteomics application for the limited number of cells
Mass spectrometry (MS)-based proteomics explores in-depth proteome, however, the analysis of cell subsets, which might be minor population, is hampered due to a multistep procedure for sample preparation and purification. Along those lines, optimization of proteomics workflow toward limited number...
ORGANISM(S): Homo Sapiens (human) 
2017-07-18 | PXD005096 | JPOST Repository
ProteomicsThiol-ene microfluidic chip for fast on-chip sample clean-up, separation and ESI mass analysis of peptides and proteins
Microfluidic devices coupled to MS promises low manufacturing costs, low sample consumption and channels with a high surface area to volume ratio and tailorable functional groups. Here, we describe a thiol-ene-based microfluidic chip that is capable of sample loading, desalting and on-chip reversed ...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021658 | Pride
Microfluidics Monolith Electrospray ionisation Mass spectrometry Reversed-phase chromatography Emitter 
ProteomicsCoupling microdroplet-based sample preparation, multiplexed isobaric labeling, and nanoflow peptide fractionation for deep proteome profiling of tissue microenvironment
Existing spatially-resolved proteomics technologies cannot provide deep proteome coverage due to limited sensitivity and poor sample recovery. Herein, we seamlessly combined laser capture microdissection with a low-volume sample processing technology that includes a microfluidic device named microPO...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-25 | MSV000093925 | MassIVE
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